BioCat Collective
Educating and Building a Broader Biocatalysis Community
Category: Biocatalysis
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Org Lett. 2026 Apr 13. doi: 10.1021/acs.orglett.6c00937. Online ahead of print. ABSTRACT Triazolopyridines are an important class of heterocycles in the pharmaceutical industry and materials sciences. In particular, [1,2,3]triazolo[1,5a]pyridines have emerged as stable and versatile diazo compound precursors for performing carbene-mediated transformations. Despite their wide range of applications in chemical synthesis, their preparation is often…
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Nat Commun. 2026 Apr 13. doi: 10.1038/s41467-026-71801-5. Online ahead of print. ABSTRACT Microbial cell factories offer a sustainable route to plant-derived natural products, but yield drift and strain degeneration persist. Growth-coupled biosynthesis can continuously enrich high producers, yet specific product-responsive biosensors remain scarce and their population-level effects are unclear. Here, we describe a rapid transcriptome-mining…
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Food Chem. 2026 Apr 8;514:149160. doi: 10.1016/j.foodchem.2026.149160. Online ahead of print. ABSTRACT Kombucha is a fermented beverage obtained from sweetened tea through the activity of a symbiotic culture of bacteria and yeast (SCOBY). While fermentation-induced transformations are well described, the role of the tea matrix in modulating these changes remains unclear. This study evaluated the…
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Enzyme Microb Technol. 2026 Apr 5;198:110874. doi: 10.1016/j.enzmictec.2026.110874. Online ahead of print. ABSTRACT β-Glucuronidase, a glycosyl hydrolase that cleaves β-glucuronic acid residues from glycosides, is widely used in the production of pharmaceutically active compounds. However, the intrinsically low thermal stability of most native enzymes restricts their industrial application. In this study, we developed an efficient…
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J Exp Bot. 2026 Apr 9:erag164. doi: 10.1093/jxb/erag164. Online ahead of print. ABSTRACT Calcium (Ca2+) is an essential mineral nutrient and a vital signaling molecule for plant growth and development. Ca2+/calmodulins (CaMs) can bind proteins, called CaM binding proteins (CBPs), to relay Ca2+ signals and modulate transcriptional activity. In this study, we demonstrate that AtCBP60b,…
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Bioelectrochemistry. 2026 Apr 8;171:109303. doi: 10.1016/j.bioelechem.2026.109303. Online ahead of print. ABSTRACT Understanding oxidative metabolism of organophosphate pesticides (OPs) in humans is critical for toxicological risk assessment. Electrode-driven bioelectrocatalysis enables enzyme catalysis without added electron donors (e.g. NADPH), providing simplified in vitro models of xenobiotic metabolism. Here we report a tandem configuration of two sequential bioelectrodes…
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ACS Chem Biol. 2026 Apr 11. doi: 10.1021/acschembio.6c00132. Online ahead of print. ABSTRACT Ufmylation is a newly identified ubiquitin-like modification of histones and plays important roles in DNA-related processes. Dissecting histone ufmylation pathways necessitates the use of chemically defined proteins to assign their structural and functional consequences; however, the preparation of ufmylated histones has not…
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Biomacromolecules. 2026 Apr 11. doi: 10.1021/acs.biomac.5c02511. Online ahead of print. ABSTRACT Resilin, an elastic protein found in insect cuticles, is exceptionally resilient because of its amino acid sequence and dityrosine cross-linking. In this study, the chemoenzymatic synthesis of resilin-mimetic polypeptides based on minimal peptide motifs incorporating glycine, proline, serine, and tyrosine residues is reported. Using…
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Appl Microbiol Biotechnol. 2026 Apr 11. doi: 10.1007/s00253-026-13811-3. Online ahead of print. ABSTRACT A novel cold-adapted and halotolerant α-amylase gene (AmyPG2) was cloned and expressed from the marine bacterium Photobacterium gaetbulicola Gung47T. The highest activity of AmyPG2 was displayed at 25 °C and pH 8.0. In addition, the residual activity of AmyPG2 remained at approximately…
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Mol Biol Rep. 2026 Apr 11;53(1):611. doi: 10.1007/s11033-026-11781-3. ABSTRACT The field of enzyme engineering has seen significant advancements with the development of fusion enzymes, which combine the properties of different enzymes through genetic manipulation. This review explores the effects of fusion on protein properties, improved catalytic efficiency, stability, and activity, as well as enhanced protein…