Int J Biol Macromol. 2026 Apr 13:152018. doi: 10.1016/j.ijbiomac.2026.152018. Online ahead of print. ABSTRACT Artificial Intelligence (AI) has emerged as a transformative tool in industrial enzyme engineering, shifting from empirical optimization to data-driven, rational modulation of enzyme activity. Recent AI methodologies extend beyond traditional structure-guided strategies, enabling the prediction of functional residues, estimation of mutational…

Nat Commun. 2026 Apr 15. doi: 10.1038/s41467-026-72050-2. Online ahead of print. ABSTRACT Enzyme-photo-coupled systems (EPCSs) offer a green strategy for producing high-value chemicals, but significant gaps remain in comparison to natural photosynthetic systems. Herein, we report an all-in-one EPCS (AiO-EPCS) by integrating photocatalytic (photocatalyst, P; electron mediator, M; electron donor, D) and biocatalytic (enzyme and…

Biodegradation. 2026 Apr 15;37(3):71. doi: 10.1007/s10532-026-10287-w. ABSTRACT Thermotolerant actinobacteria are important because they thrive in harsh environments and efficiently degrade agricultural residues, producing valuable enzymes for various applications. In this study, a thermophilic Streptomyces griseorubens NBR14 strain was used to produce cellulase from wheat straw via submerged fermentation. The production was optimized using a Box-Behnken…

Adv Sci (Weinh). 2026 Apr 15:e13600. doi: 10.1002/advs.202513600. Online ahead of print. ABSTRACT Orthogonal translation systems (OTSs) enable site-specific incorporation of non-canonical amino acids (ncAAs) and are central to genetic code expansion. Current engineering strategies typically rely on hyperstable aminoacyl tRNA synthetase (aaRS) scaffolds to tolerate destabilizing mutations required for substrate diversification. Here, we introduce…

Adv Biotechnol (Singap). 2026 Apr 15;4(2):14. doi: 10.1007/s44307-026-00104-z. ABSTRACT Enzymatic depolymerization of polyethylene terephthalate (PET), the world’s most widely used polyester, in seawater at ambient temperature offers a promising and energy-efficient route for freshwater-free plastic recycling. While a number of PET hydrolases have been reported in recent years, their potential under saline conditions remains largely…

Angew Chem Int Ed Engl. 2026 Apr 15:e3445735. doi: 10.1002/anie.3445735. Online ahead of print. ABSTRACT Chiral silicon-stereogenic organosilanes are finding increasingly widespread applications in pharmaceutical science and biomedical materials. However, the enzymatic construction of silicon chiral centers remains underdeveloped. Here, we report the integration of thiamine diphosphate (ThDP)-dependent radical biocatalysis and mediated electrochemical oxidation to…

Colloids Surf B Biointerfaces. 2026 Apr 10;265:115704. doi: 10.1016/j.colsurfb.2026.115704. Online ahead of print. ABSTRACT Enzyme immobilization is crucial for industrial biocatalysis but is fundamentally limited by an inherent activity-stability trade-off, where rigid protective supports often compromise catalytic efficiency through severe diffusion barriers or denaturation. To address this challenge, we developed a bioinspired “nanoarmor” via interfacial…

Int J Biol Macromol. 2026 Apr 12:151983. doi: 10.1016/j.ijbiomac.2026.151983. Online ahead of print. ABSTRACT 4,5-DOPA extradiol dioxygenase (DODA) is a key enzyme in the catabolism of aromatic compounds and holds significant promise for biocatalysis applications. This study presents the first successful immobilization of recombinant DODA with a C-terminal His-tag, paving the way for in-vitro biocatalytic…

Commun Chem. 2026 Apr 14. doi: 10.1038/s42004-026-01986-9. Online ahead of print. ABSTRACT TraH, a fungal decarboxylase from Penicillium crustosum, belongs to the isopenicillin N synthase (IPNS) subfamily of non-heme iron/α-ketoglutarate (αKG)-dependent enzymes. IPNS enzymes are characterized by N- and C-terminal insertions that can reshape the active site. However, the functional and mechanistic roles of these…

Bioorg Chem. 2026 Apr 11;176:109824. doi: 10.1016/j.bioorg.2026.109824. Online ahead of print. ABSTRACT Deoxycholic acid (DCA) is a key secondary bile acid with multifaceted physiological functions and therapeutic applications, most notably as the active ingredient in ATX-101, the only approved pharmaceutical for moderate-to-severe submental fat. Conventional chemical synthesis of DCA, however, relies on hazardous reagents and…