BioCat Collective
Educating and Building a Broader Biocatalysis Community
Category: Biocatalysis
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Bioresour Technol. 2026 May 21:134952. doi: 10.1016/j.biortech.2026.134952. Online ahead of print. ABSTRACT Raspberry ketone synthase is the key enzyme for raspberry ketone biosynthesis, yet its activity and thermostability remain suboptimal for industrial use. By combining computational and deep-learning-guided design, we obtained a Rubus idaeus raspberry ketone synthase (RiRZS1) mutant M3 with 12.06-fold higher relative activity…
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Chemphyschem. 2026 May 27;27(10):e70414. doi: 10.1002/cphc.70414. ABSTRACT Serine β – $beta -$ lactamases are bacterial enzymes that cause antibiotic resistance by rapid hydrolysis of β – $beta -$ lactam antibiotics. Development of efficient covalent inhibitors for serine β $beta$ -lactamases requires an understanding of the factors that can increase the free energy barrier for hydrolysis…
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ACS Chem Biol. 2026 May 22. doi: 10.1021/acschembio.6c00013. Online ahead of print. ABSTRACT Thioesterase (TE) domains catalyze peptide release and macrocyclization during nonribosomal peptide biosynthesis, typically via catalytic serine. In contrast, MacB-TE from macolacin biosynthesis employs a rare cysteine nucleophile, raising questions regarding the catalytic requirements of cysteine-dependent peptide cyclases. Here, we report the biochemical…
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Zool Res. 2026 May 22:1. doi: 10.24272/j.issn.2095-8137.2025.527. Online ahead of print. NO ABSTRACT PMID:42169389 | DOI:10.24272/j.issn.2095-8137.2025.527
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J Agric Food Chem. 2026 May 21. doi: 10.1021/acs.jafc.6c00983. Online ahead of print. ABSTRACT The tea plant is known as a fluoride hyperaccumulator, with excessive fluoride accumulating in its leaves and posing a potential threat to human health. Previous studies have pinpointed that certain genes contributed to fluoride accumulation in tea plants; however, the role…
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Bioorg Med Chem Lett. 2026 May 20:130691. doi: 10.1016/j.bmcl.2026.130691. Online ahead of print. ABSTRACT pH-labile linkers have garnered significant attention in biomedical applications, particularly in designing the stimuli-responsive carriers for targeted drug delivery systems. In the present work, we have synthesized G1-monoacrylate from G1-diacrylate using a chemoenzymatic approach, followed by the synthesis of glycerol-based β-thiopropionate…
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Int J Biol Macromol. 2026 May 20:152585. doi: 10.1016/j.ijbiomac.2026.152585. Online ahead of print. ABSTRACT Lignocellulosic biomass (LCB), a second-generation feedstock for cellulosic ethanol production, requires efficient pretreatment. This step is critical in biorefinery processes. During enzymatic hydrolysis, lignin limits cellulase accessibility, whereas β-glucosidase (BGL) functions as a rate-limiting enzyme. This review systematically summarises lignin-targeted pretreatment…
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Spectrochim Acta A Mol Biomol Spectrosc. 2026 May 20;361:128086. doi: 10.1016/j.saa.2026.128086. Online ahead of print. ABSTRACT The accurate determination of phosphate (Pi) content in dairy products is essential for safeguarding consumer health and maintaining product quality standards. Herein, a sensing platform that integrates synergistic biomimetic enzyme catalysis with photoluminescence (PL) enhancement was developed for ratiometric…
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Eur J Med Chem. 2026 May 17;315:118958. doi: 10.1016/j.ejmech.2026.118958. Online ahead of print. ABSTRACT In this work, eight novel chromeno-dipyrimidine derivatives were designed and synthesized through a green, catalyst-free one-pot hemisynthetic approach starting from the natural perillaldehyde, barbituric acid and substituted anilines. All prepared polycyclic heterocycles were isolated in high yields (74-94%), and were fully…
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Food Sci Nutr. 2026 May 19;14(5):e71818. doi: 10.1002/fsn3.71818. eCollection 2026 May. ABSTRACT The use of agricultural waste offers an eco-friendly and sustainable way to synthesize functional nanomaterials. In this study, silver oxide nanoparticles (AgO-NPs) were synthesized by using peel extracts from Citrus deliciosa (CD) and Citrus nobilis (CN) through a green method. The bioactive compounds…