Bioprocess Biosyst Eng. 2026 May 25. doi: 10.1007/s00449-026-03353-3. Online ahead of print. ABSTRACT This study investigates the atypical kinetics of an (S)-selective oxidoreductase during the oxidation of (S)-rhododendrol to raspberry ketone. The enzyme kinetics exhibit a biphasic pattern: one follows classic Michaelis-Menten kinetics, while the other shows substrate inhibition, which is consistent with the presence…

Chembiochem. 2026 May 27;27(10):e70381. doi: 10.1002/cbic.70381. ABSTRACT Coenzyme regeneration is essential for oxidoreductase-based biocatalysis, yet integrating enzymatic and photocatalytic systems remains challenging due to ROS-mediated enzyme inactivation. Here, we developed a metabolic-inspired compartmentalization strategy that enables sustainable NAD+/NADH cycling by mimicking the spatial organization of cellular redox metabolism. Glucose dehydrogenase (GDH) was encapsulated within silica…

Appl Biochem Biotechnol. 2026 May 25. doi: 10.1007/s12010-026-05739-1. Online ahead of print. ABSTRACT This review comprehensively examines the application and molecular modification of enzymes in the textile degumming of plant-based natural fibers. Traditional chemical and physical degumming methods are energy-intensive and environmentally detrimental, having negative impact on fibers’ mechanical properties and performance. In contrast, enzymatic…

J Exp Bot. 2026 May 23:erag247. doi: 10.1093/jxb/erag247. Online ahead of print. ABSTRACT Suberin polymers deposited in roots form barriers for the transport of water and mineral nutrients and provide primary protection against pathogen attack. Soybean (Glycine max) is one of the most widely grown crops for feeding humans and livestock globally. However, the regulatory…

Enzyme Microb Technol. 2026 May 20;199:110904. doi: 10.1016/j.enzmictec.2026.110904. Online ahead of print. ABSTRACT Keratin is an abundant structural fibrous protein and extremely recalcitrant biopolymer. β-Keratin is the major constituent of feathers, which, due to the widespread poultry industry, has become a major waste product. Biotechnological upcycling of feather waste has gained interest as various bacteria…

Methods Enzymol. 2026;730:75-90. doi: 10.1016/bs.mie.2025.11.001. Epub 2025 Nov 27. ABSTRACT Type III polyketide synthases (T3PKSs) are a versatile family of enzymes that generate structurally diverse natural products with ecological and clinical importance. A major contributor to their broad product scope is their inherent substrate promiscuity. Accurately predicting the substrate scope of T3PKSs supports their application…

Microb Cell Fact. 2026 May 23. doi: 10.1186/s12934-026-03014-w. Online ahead of print. ABSTRACT Aromatics are important building blocks for polymers, pharmaceuticals, and advanced materials, but their current production relies on petrochemical processes. Biotechnological de novo production from renewable bio-based feedstocks with microbial cell factories provides a sustainable alternative. In this study, we enhanced 4-coumarate production…

Int J Biol Macromol. 2026 May 21:152642. doi: 10.1016/j.ijbiomac.2026.152642. Online ahead of print. ABSTRACT Organisms maintain protein structure and function under environmental stress conditions by accumulating small organic molecules known as osmolytes. Mixtures of osmolytes can form natural deep eutectic solvents (NADESs), which have attracted attention as environmentally friendly media for biocatalysis; however, the effect…

Biotechnol Adv. 2026 May 21:108929. doi: 10.1016/j.biotechadv.2026.108929. Online ahead of print. ABSTRACT As a major class of petroleum hydrocarbons, n-alkanes are important resources. Advances in biotechnology have led to the discovery of microorganisms capable of metabolizing n-alkanes. Liquid n-alkanes have moderate carbon chain length and good biocompatibility, allowing their complete mineralization into carbon dioxide and…

J Biotechnol. 2026 May 21:S0168-1656(26)00163-X. doi: 10.1016/j.jbiotec.2026.05.010. Online ahead of print. ABSTRACT The synthesis of key chiral intermediates via biocatalysis is of great importance in pharmaceutical manufacturing. In this study, we engineered a phenylacetone monooxygenase from Thermobifida fusca to enhance its catalytic activity for the synthesis of methyl (2S)-5-chloro-2-hydroxy-1-oxo-2,3-dihydro-1H-indene-2-carboxylate, a crucial precursor of the insecticide…