Protein Sci. 2026 Jun;35(6):e70633. doi: 10.1002/pro.70633. ABSTRACT Water plays a critical role in the hydrolysis of antibiotics by New Delhi metallo-β-lactamases (NDMs). The reaction proceeds through a nucleophilic attack on the β-lactam ring, followed by cleavage of the C-N bond and formation of an anionic nitrogen intermediate. This intermediate is then protonated by a water…

Protein Sci. 2026 Jun;35(6):e70632. doi: 10.1002/pro.70632. ABSTRACT A growing number of enzymes demonstrate deviations from typical linear kinetic temperature dependencies as modeled by the Arrhenius and Eyring equations. These deviations may manifest as biphasic temperature dependencies consisting of two individually linear regions meeting at a break point, which suggests a change of conformation or dynamics…

Integr Zool. 2026 May 18. doi: 10.1111/1749-4877.70127. Online ahead of print. ABSTRACT Sex differences in behavior and functional traits are often attributed to differences in mating effort intensity, but the role of sex-specific parental demands remains poorly understood. Using the jumping spider Toxeus maxillosus-where males engage in mate searching and courtship without providing parental care,…

Iran J Biotechnol. 2026 Apr 1;24(2):e4184. doi: 10.30498/ijb.2025.532876.4184. eCollection 2026 Apr. ABSTRACT BACKGROUND: The increasing environmental impact of food waste, especially starch-rich residues, necessitates sustainable biodegradation methods to reduce pollution and resource wastage. OBJECTIVES: This study aimed to isolate α-amylase-producing bacteria from saline-rich organic soils in Jeddah, Saudi Arabia, and assess their effectiveness in degrading…

J Am Chem Soc. 2026 May 17. doi: 10.1021/jacs.6c01275. Online ahead of print. ABSTRACT While biocatalysis and electrochemistry each offer unique advantages, such as enzyme selectivity and traceless electron use, their integration for unlocking new enzyme function remains limited. Here, we report a new-to-nature electroenzymatic desymmetrization strategy that enables the enantiodivergent synthesis of biaryls bearing…

Bioelectrochemistry. 2026 May 14;171:109329. doi: 10.1016/j.bioelechem.2026.109329. Online ahead of print. ABSTRACT Light-driven biohybrid systems that couple semiconductor nanocrystals with enzymes offer a promising strategy for solar-to-chemical energy-conserving reduction reactions, yet are often limited by inefficient hole scavenging. Hole scavenging is critical for maintaining charge separation in the light-absorbing electron donor molecule and, thus, for sustaining…

Sci Rep. 2026 May 17. doi: 10.1038/s41598-026-53113-2. Online ahead of print. ABSTRACT In this study, a laccase-mediator system utilizing caffeic acid as a mediator was developed for the green synthesis of benzimidazole derivatives. The reactions were carried out under mild conditions at room temperature, using citrate buffer as the reaction medium and aerial oxygen as…

Phytochemistry. 2026 May 15:114957. doi: 10.1016/j.phytochem.2026.114957. Online ahead of print. ABSTRACT Hydroxycinnamic acid amides (HCAAs) featuring spermine or spermidine represent a major class of specialized metabolites in goji berries (Lycium spp.) and exhibit diverse pharmacological activities. Acylation is essential for HCAAs biosynthesis, yet the underlying enzymatic mechanisms in goji berries remain unclear. In this study,…

J Chromatogr B Analyt Technol Biomed Life Sci. 2026 May 12;1280:125111. doi: 10.1016/j.jchromb.2026.125111. Online ahead of print. ABSTRACT Therapeutic F(ab’)2 fragments derived from polyclonal equine antisera remain essential emergency biologics for addressing snake envenomation, tetanus, and viral infections. Traditional production methods rely on pepsin digestion, which is limited by non-specific proteolysis, harsh acidic conditions (pH…