J Mol Biol. 2026 Jun 2:169890. doi: 10.1016/j.jmb.2026.169890. Online ahead of print. ABSTRACT SixA is a phosphatase that dephosphorylates phosphohistidine in proteins involved in signaling in bacteria. Using NMR spectroscopy, the interaction of SixA was structurally characterized with one of its functional substrates, the phosphocarrier protein NPr, which shuttles a phosphate group between components of…

ACS Synth Biol. 2026 Jun 3. doi: 10.1021/acssynbio.6c00129. Online ahead of print. ABSTRACT Azomycin, as a representative example of valued nitro compounds, suffers from harsh reaction conditions and low yields in its traditional synthesis methods. Biocatalytic synthesis using N-oxygenase offers a green alternative but is constrained by the enzyme’s limited efficiency and stability. To overcome…

Comput Struct Biotechnol J. 2026 Jun 1;35(1):0105. doi: 10.34133/csbj.0105. eCollection 2026. ABSTRACT Artificial intelligence (AI) has transformed protein engineering by leveraging deep learning, protein language models, and knowledge graphs to decode relationships between sequence, structure, and function. Models like AlphaFold2 achieve near-experimental accuracy in structure prediction, while transformer-based language models facilitate de novo sequence design…

RSC Chem Biol. 2026 Jun 1. doi: 10.1039/d6cb00069j. Online ahead of print. ABSTRACT Glycosyl transfer constitutes a key step in the functionalisation of bioactive molecules, including proteins and natural products. In vitro studies to dissect glycosyltransferase catalysis are often challenging due to poor enzyme solubility, enzyme instability, and the requirement of advanced analytical setups. Herein,…

Biochem Biophys Res Commun. 2026 May 26;827:154012. doi: 10.1016/j.bbrc.2026.154012. Online ahead of print. ABSTRACT (S)-selective ω-transaminases (S-ωTAs) are PLP-dependent enzymes widely employed in biocatalysis for the stereoselective amination of prochiral ketones, yielding enantiopure (S)-amines. Although their stereochemical preference is well established, the structural basis of keto-acceptor recognition and active-site flexibility remains poorly understood. Here, we…

Int J Biol Macromol. 2026 Jun 1:152842. doi: 10.1016/j.ijbiomac.2026.152842. Online ahead of print. ABSTRACT Lu’an Guapian is a traditional Chinese green tea renowned for its special roasting process, while its functionalities rarely been revealed. This study aimed to explore the immunomodulatory effects of Guapian polysaccharides (LGP) on cyclophosphamide (CTX) -induced immunosuppressed mice. LGP with main…

Biotechnol Adv. 2026 Jun 1:108943. doi: 10.1016/j.biotechadv.2026.108943. Online ahead of print. ABSTRACT Selective oxygenation reactions are highly valuable for the pharmaceutical industry, as they provide new pharmacokinetic and physicochemical properties to a compound. Yet, conventional methods often suffer from low yields, poor selectivity, and numerous steps. In that context, P450 enzymes appear as an attractive…

Protein Sci. 2026 Jul;35(7):e70647. doi: 10.1002/pro.70647. ABSTRACT Soybean lipoxygenase (GmLOX1), a plant 13-LOX, has long been considered a model protein for non-trivial, quantum hydrogen tunneling in enzyme catalysis. Hydrogen tunneling mechanisms have also been confirmed for LOXs across mammalian, fungal, and bacterial kingdoms. Notably, GmLOX1 exhibits a low activation energy (Ea) for catalysis relative to…

Adv Mater. 2026 Jun 1:e73560. doi: 10.1002/adma.73560. Online ahead of print. ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA)-infected diabetic foot ulcers (DFUs) remain refractory to healing owing to persistent inflammation, hyperglycemia, and impaired tissue regeneration. Current antimicrobial strategies primarily eliminate viable bacteria but overlook pathogen-associated molecular patterns (PAMPs) released upon bacterial death, which sustain NF-κB/NLRP3 activation and…