Biotechnol J. 2026 Jun;21(6):e70253. doi: 10.1002/biot.70253. ABSTRACT Oral probiotics, as living therapeutics, hold considerable promise for modulating host health and preventing diseases, with substantial potential for industrial and clinical applications. However, their efficacy is substantially limited by gastrointestinal degradation and inadequate colonization, and the oversimplified linear correlation between strain survival and therapeutic efficacy, further hinder…

J Am Chem Soc. 2026 Jun 1. doi: 10.1021/jacs.6c05047. Online ahead of print. ABSTRACT The asymmetric construction of highly congested polycyclic architectures remains a formidable challenge in chemical synthesis. Biocatalysis offers exquisite stereocontrol, yet the repertoire of enzyme-catalyzed cycloadditions remains notably limited. Here, we elucidate the intriguing oxidative [3 + 2] cycloaddition capability of a…

J Microbiol Biotechnol. 2026 May 25;36:e2604021. doi: 10.4014/jmb.2604.04021. ABSTRACT Cells are extraordinary biochemical systems that have evolved over billions of years into highly sophisticated units of life. Bottom-up synthetic biology seeks to reconstruct cell-like systems from non-living molecular components, producing artificial cells that capture essential cellular features while bypassing the complexity and fragility of living…

Synth Syst Biotechnol. 2026 May 21;14:280-309. doi: 10.1016/j.synbio.2026.04.022. eCollection 2026 Dec. ABSTRACT The eukaryotic cell-free protein synthesis (CFPS) system, endowed with intrinsic post-translational modification capabilities and a complex molecular chaperone network, efficiently synthesizes functional proteins with correct conformation and biological activity. This effectively compensates for the structural limitations of prokaryotic systems in expressing complex eukaryotic…

Chembiochem. 2026 Jun 15;27(11):e70404. doi: 10.1002/cbic.70404. ABSTRACT Halogenation reactions are essential for producing many fine and bulk chemicals. Vanadium-dependent chloroperoxidases (VCPOs) are promising biocatalysts for a more sustainable production of halogenated products because they are cofactor independent, use hydrogen peroxide (H2O2) as oxygen donor, tolerate high H2O2 concentrations, and catalyze all halogenations except fluorination. Despite…

Ecotoxicol Environ Saf. 2026 May 30;319:120322. doi: 10.1016/j.ecoenv.2026.120322. Online ahead of print. ABSTRACT Endocrine-disrupting estrogens, such as 17β-estradiol (E2) and bisphenol A (BPA), are commonly found in agroecosystems, posing substantial risks to crop productivity and public health. While laccase-induced polymerization transfer is an effective strategy for estrogen remediation, the interactive effects of living crop root-released…

Appl Microbiol Biotechnol. 2026 May 30;110(1):164. doi: 10.1007/s00253-026-13890-2. ABSTRACT Neglected tropical diseases and the increasing prevalence of antibiotic resistance in nosocomial infections represent a major challenge in medicine. Intensive research into bioactive compounds has identified the myxobacterial natural product aurachin D as promising drug candidate due to its potent antibiotic and antiprotozoal properties. The biosynthesis…

Bioresour Technol. 2026 May 28:135013. doi: 10.1016/j.biortech.2026.135013. Online ahead of print. ABSTRACT Microfluidic biocatalytic reactors have drawn considerable interest in recent years, but their high production costs hinder scalability for industrial use. It is urgent to develop a low-cost and green fluid reactor. Here, inspired by the structure of natural bamboo, a cellulose-based bionic microfluidic…