Enzyme Microb Technol. 2026 Jun 1;200:110915. doi: 10.1016/j.enzmictec.2026.110915. Online ahead of print. ABSTRACT Enzymes play a crucial role in metabolism. The rate at which enzyme sequence data is emerging far exceeds the rate at which we can measure the associated catalytic constants, posing a significant problem for the advancement of the fields of metabolic engineering…

Int J Pharm. 2026 Jun 5:127047. doi: 10.1016/j.ijpharm.2026.127047. Online ahead of print. ABSTRACT Acute lung infection (ALI) represents a major global health challenge with high morbidity and mortality. The current clinical approach primarily relies on antibiotic therapy, yet the therapeutic efficacy is severely limited by poor drug targeted delivery. Here, we developed neutrophil-hijacking pH/enzyme dual-responsive…

Extremophiles. 2026 Jun 6;30(1):21. doi: 10.1007/s00792-026-01433-6. ABSTRACT Glaciozyma antarctica is an Antarctic psychrophilic yeast providing an excellent eukaryotic model for dissecting molecular mechanisms of cold adaptation. Yet, its extracellular protein repertoire, the interface between the cell and extreme surroundings, remains unexplored. Here, we present the first integrated bioinformatics-proteomics secretome map of G. antarctica, linking computational…

Adv Mater. 2026 Jun 5:e20108. doi: 10.1002/adma.202520108. Online ahead of print. ABSTRACT Reconstruction of functional microvascular networks, particularly at the capillary scale, remains a critical bottleneck for the integration of tissue-engineered grafts with host tissues. Here, we report a photothermal ablation blotting (PAB) strategy for engineering perfusable, robust, and hierarchically branched microvascular network within a…

Bioorg Chem. 2026 Jun 1;180:110064. doi: 10.1016/j.bioorg.2026.110064. Online ahead of print. ABSTRACT Biocatalysis has emerged as an essential green manufacturing approach, enabling sustainable and efficient synthesis across the pharmaceutical, agricultural, and food industries. Beyond its fundamental significance in elucidating molecular evolution, ancestral sequence reconstruction (ASR) has also proven to be a powerful technology for engineering…

bioRxiv [Preprint]. 2026 May 26:2026.05.21.726972. doi: 10.64898/2026.05.21.726972. ABSTRACT Proteins function through a complex interplay of structural and biochemical properties, and mutations can reshape these properties to generate fitness landscapes spanning multiple functional objectives. A central challenge in protein engineering is the need to simultaneously optimize multiple properties. In biocatalysis, for example, practical enzyme development routinely…

ACS Sustain Chem Eng. 2026 May 20;14(21):9741-9754. doi: 10.1021/acssuschemeng.5c13215. eCollection 2026 Jun 1. ABSTRACT Enzymatic depolymerization of polyethylene terephthalate (PET) is a promising alternative to traditional recycling showing promise in addressing issues with high energy demands and challenges associated with mixed-waste streams. While engineered, thermostable PETase variants exist, achieving high yields under industrially relevant process…

PLoS One. 2026 Jun 4;21(6):e0346328. doi: 10.1371/journal.pone.0346328. eCollection 2026. ABSTRACT Fraxinus xanthoxyloides Wall. ex DC (Family-Oleaceae) is a tiny tree found in arid highlands, often referred to as “Afghan ash”. This study aimed to explore F. xanthoxyloides bark extract as a potential anti-diabetic agent by conducting GC-MS analysis along with in vitro and in vivo…

ChemSusChem. 2026 Jun 15;19(11):e70784. doi: 10.1002/cssc.70784. ABSTRACT The continuous increase in textile waste, coupled with insufficient recycling and reuse strategies, has led to a growing issue of underutilized raw materials. Mixed textiles account for approximately 50% of global textile waste and commonly consist of tightly woven cotton blended with synthetic fibers, which complicated material separation…

Synth Syst Biotechnol. 2026 May 25;14:359-367. doi: 10.1016/j.synbio.2026.04.039. eCollection 2026 Dec. ABSTRACT d-Arabinitol is a high-value platform chemical widely applied in food, cosmetic, and pharmaceutical industries, yet conventional production suffers from low conversion efficiency, prolonged cycles, and considerable environmental hazards. Herein, an artificial in vitro enzymatic cascade was developed consisting of Thermotoga maritima ribose-5-phosphate isomerase…