Anal Sci Adv. 2026 May 26;7(1):e70091. doi: 10.1002/ansa.70091. eCollection 2026 Jun. ABSTRACT Carbon dioxide (CO2) sequestration is a key strategy for mitigating climate change, and biocatalytic approaches offer sustainable, cost-effective alternatives to chemical and physical methods. Carbonic anhydrases (CAs) efficiently catalyse the reversible hydration of CO2 to bicarbonate, yet their large-scale application is constrained by…

ACS Omega. 2026 May 18;11(21):30456-30463. doi: 10.1021/acsomega.5c09946. eCollection 2026 Jun 2. ABSTRACT Ionic liquids (ILs) and deep eutectic solvents (DESs) are currently of great interest due to their highly interesting physicochemical properties; they were studied throughout many chemical and biological approaches, and they were used intensively in biocatalysis and biomedicine.In the present study, ten synthesized…

Int J Biol Macromol. 2026 Jun 6:152931. doi: 10.1016/j.ijbiomac.2026.152931. Online ahead of print. ABSTRACT Lipases from halotolerant bacteria represent promising biocatalysts for industrial applications due to their stability under extreme conditions. In this study, a halotolerant strain Salegentibacter sp. MHS1-6 was isolated from Western Pacific deep-sea sediments, and its lipase was characterized for the first…

J Comput Chem. 2026 Jun 15;47(16):e70434. doi: 10.1002/jcc.70434. ABSTRACT The glycine N-methyltransferase (GNMT) reaction was examined using an integrated workflow combining molecular dynamics (MD), quantum mechanical (QM) cluster calculations, and machine learning (ML) analysis. Instead of relying on a single crystal-like conformation, multiple MD simulations were used to sample diverse reactant (SAM + glycine bound…

Small. 2026 Jun 7:e00066. doi: 10.1002/smll.202600066. Online ahead of print. ABSTRACT Continuous-flow biocatalysis enables sustainable synthesis but suffers from diminished enzyme-substrate interactions under convective transport. Nanoconfinement addresses the challenge, yet the lack of tunable, flow-compatible nanoreactors has limited exploration of extreme confinement matching the enzyme dimension under flow. Here, we reveal an enzyme-scale confinement regime…

ACS Synth Biol. 2026 Jun 7. doi: 10.1021/acssynbio.6c00244. Online ahead of print. ABSTRACT l-Cysteine is a high-value sulfur-containing amino acid indispensable for industrial and medical applications. However, the production of l-cysteine is limited by the low sulfur assimilation efficiency and complex regulatory networks of native pathways. Here, we report the design and construction of a…

Chembiochem. 2026 Jun 15;27(11):e70424. doi: 10.1002/cbic.70424. ABSTRACT Industrial biocatalysts are strongly influenced by their physicochemical environment, yet systematic studies on how metals and macromolecular crowding influence metal-independent esterases remain scarce. Here, Pseudomonas fluorescens esterase I (PFE), an α/β-hydrolase, is employed to evaluate how divalent cations and molecular crowding modulate catalysis and structural integrity. PFE retained…

J Agric Food Chem. 2026 Jun 8. doi: 10.1021/acs.jafc.6c04510. Online ahead of print. ABSTRACT Ginsenoside Rf, a rare protopanaxatriol-type ginsenoside derived from Panax ginseng, exhibits notable neuroprotective, anti-inflammatory, and antitumor properties. However, its low natural abundance has impeded large-scale production. Here, we reprogrammed the sugar donor specificity of PnUGT94Q39 from UDP-xylose to UDP-glucose, thereby enabling…

Biomed Pharmacother. 2026 Jun 6;200:119589. doi: 10.1016/j.biopha.2026.119589. Online ahead of print. ABSTRACT Chalcones are tunable electrophiles whose bioactivity depends on aryl substitution and membrane interactions. We profiled eight 4′-hydroxychalcones with differing in methoxy topology on ring B in primary human PBMCs (24 h, XTT) and canine lymphoma/leukemia cell lines (CLBL-1, CLB70; 72 h, MTT), integrating…