BioCat Collective
Educating and Building a Broader Biocatalysis Community
Category: Biocatalysis
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Chembiochem. 2026 Jun 26;27(12):e70438. doi: 10.1002/cbic.70438. ABSTRACT 4-Oxalocrotonate tautomerase (4-OT) is a promising scaffold for asymmetric biocatalysis, but conventional 4-OT-catalyzed aldol reactions focus solely on aldehyde substrate activation. Herein, we report the directed evolution of a tandem-fused 4-OT enzyme that achieves efficient activation of ketone substrates for enantioselective aldol reactions with substituted benzaldehydes. The engineered…
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Colloids Surf B Biointerfaces. 2026 Jun 21;267:115923. doi: 10.1016/j.colsurfb.2026.115923. Online ahead of print. ABSTRACT Burn wounds infected with Staphylococcus aureus (S. aureus) are often characterized by excessive early inflammation, which leads to persistent oxidative stress, pain, and delayed healing. To overcome the limitations of conventional photosensitizers, which may induce reactive oxygen species (ROS) overload and…
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Mikrochim Acta. 2026 Jun 23;193(7):491. doi: 10.1007/s00604-026-08197-y. ABSTRACT A high-performance, flexible surface-enhanced Raman scattering (SERS) sensing platform designed to detect key hyperuricemia (HUA) biomarkers in non-invasive biofluids is presented. The platform employs a bimetallic nanocomposite hydrogel fabricated via a semi-interpenetrating polymer network, wherein hydroxyethyl cellulose-stabilized gold nanorods are integrated with in situ-reduced silver nanoparticles from…
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Bioresour Technol. 2026 Jun 23:135231. doi: 10.1016/j.biortech.2026.135231. Online ahead of print. ABSTRACT Biofilms catalysis has broad application prospects in the biocatalysis due to its high cellular stability, strong reusability, and operational simplicity. However, the naturally limited biofilm-forming ability of Escherichia coli restricts its performance in catalytic processes. In this study, we developed a rational engineering…
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Bioresour Technol. 2026 Jun 23:135229. doi: 10.1016/j.biortech.2026.135229. Online ahead of print. ABSTRACT Amino acid oxidases (AAOs: EC 1.4.3.X, D- and l-forms) have attracted significant interest as promising biocatalysts for the synthesis of high-value chiral compounds, including natural and non-natural amino acids. These FAD-dependent enzymes catalyze the oxidative deamination of amino acids to corresponding imino acids…
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J Am Chem Soc. 2026 Jun 23. doi: 10.1021/jacs.6c06355. Online ahead of print. ABSTRACT 6-sulfo Lewis x-related glycan epitopes, including 6-sulfo Lewis x and its sialylated form, sialyl 6-sulfo Lewis x, constitute an important class of carbohydrate determinants that are widely expressed in human cells. Elucidating the biological roles and structure-function relationships of these glycans…
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Appl Biochem Biotechnol. 2026 Jun 23. doi: 10.1007/s12010-026-05790-y. Online ahead of print. ABSTRACT In this study, divalent metal ions (Zn²⁺, Cu²⁺, and Mn²⁺) were employed for the in situ synthesis of highly stable hybrid nanoflowers (tAG-NFs) to immobilize transglycosylating α-glucosidase (tAG) for isomaltooligosaccharide (IMO) production. Characterization via SEM, FTIR, and XRD confirmed the successful formation…
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J Am Chem Soc. 2026 Jun 22. doi: 10.1021/jacs.6c06320. Online ahead of print. ABSTRACT Glycoproteins often exist in nature as heterogeneous glycoforms, making it difficult to obtain pure homogeneous samples for studying the roles of specific N-glycans within a glycoprotein. Despite various chemical and enzymatic methods developed for the synthesis of N-glycans, access to highly…
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Adv Sci (Weinh). 2026 Jun 22:e76240. doi: 10.1002/advs.76240. Online ahead of print. ABSTRACT The dynamic interplay between bacteria and host cancer cells plays a critical role in tumor microenvironment modulation, bacterial pathogenesis, and potential oncotherapy applications. However, traditional methods often fail to capture transient or spatially restricted molecular interactions at the bacteria-cancer cell interface. Proximity…
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ACS Omega. 2026 Jun 1;11(23):34659-34667. doi: 10.1021/acsomega.6c03268. eCollection 2026 Jun 16. ABSTRACT Inhibition of enzyme catalysis is critical for regulating cellular metabolism. Gentisate and salicylate 1,2-dioxygenases (GDO and SDO) are primary enzymes in the key gentisic pathway, which perform O2-dependent degradation of aromatic compounds with the aid of a ferrous cofactor. Here, we report on…