Adv Sci (Weinh). 2026 Jun 22:e76240. doi: 10.1002/advs.76240. Online ahead of print.
ABSTRACT
The dynamic interplay between bacteria and host cancer cells plays a critical role in tumor microenvironment modulation, bacterial pathogenesis, and potential oncotherapy applications. However, traditional methods often fail to capture transient or spatially restricted molecular interactions at the bacteria-cancer cell interface. Proximity labeling has emerged as a promising technology for capturing the interaction between bacteria and host-cancer cell. Photocatalytic proximity labeling is more efficient, faster, and higher in resolution than enzyme-catalyzed proximity labeling. Here, we employ photoactivatable proximity labeling technology-the “Ru-1O2-hydrazide” system to rapidly capture the interaction between bacteria and host cancer cells. This system is using the singlet oxygen (1O2) mechanism with biotin hydrazide by anchoring the photosensitizer Ru(bpy)3 2+ on the bacteria surface to efficiently and discriminatively capture the bacteria-host cancer cell interactions (BHIs). Furthermore, we established a quantitative strategy based on the “Ru-1O2-hydrazide” system to characterize bacteria-host cell interaction strength. This strategy allows for systematic evaluation of drug effects on BHIs, offering novel insight into pharmaceutical modulation of bacteria-host cancer cell crosstalk.
PMID:42325121 | DOI:10.1002/advs.76240