BioCat Collective
Educating and Building a Broader Biocatalysis Community
Category: Biocatalysis
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J Phys Chem Lett. 2026 Jun 26. doi: 10.1021/acs.jpclett.6c01422. Online ahead of print. ABSTRACT Vibrational energy transfer (VET) in enzymes has been discussed in the context of enzyme catalysis. A key point for experimentally investigating VET is to position suitable vibrational energy sensors in the delicate active sites of enzymes without disturbing their native state.…
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N Biotechnol. 2026 Jun 26:S1871-6784(26)00083-X. doi: 10.1016/j.nbt.2026.06.008. Online ahead of print. ABSTRACT UDP-GlcNAc and UDP-GalNAc are well-demanded nucleotide sugars and serve as precursors for the enzymatic synthesis of N- and O-glycans as well as glycosaminoglycans. Although the enzymatic synthesis cascade has been extensively studied, limitations remain for an efficient large-scale production. Unfavorable inhibitions by intermediate…
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Phys Chem Chem Phys. 2026 Jun 26. doi: 10.1039/d6cp01082b. Online ahead of print. ABSTRACT An improved understanding of the cooperative and antagonistic impacts of ionic liquids (ILs) and nanoparticles (NPs) on proteins is necessary for the progress of biocatalysis, biosensing and nanobiotechnology. Here, we, for the first time, analyze an important aspect of the mechanism…
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Angew Chem Int Ed Engl. 2026 Jun 26:e5406475. doi: 10.1002/anie.5406475. Online ahead of print. ABSTRACT Aqueous monoethanolamine (MEA) solutions can absorb CO2 from industrial point sources via amine scrubbing. Mechanistically, CO2 diffuses in and reacts with MEA or hydroxide to form a mixture of carbamate and carbonate/bicarbonate. Subsequently, CO2 is released for storage or utilization…
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Chemoenzymatic labeling of RNA using terminal uridylyl transferase and bioorthogonal click chemistry
Methods Enzymol. 2026;731:223-252. doi: 10.1016/bs.mie.2026.05.007. Epub 2026 Jun 1. ABSTRACT The ability of native and engineered nucleic acid-processing enzymes to incorporate clickable nucleotide substrates has greatly advanced bioorthogonal labeling of nucleic acids, overcoming the limitations of conventional solid-phase oligonucleotide (ON) synthesis. In this chemoenzymatic approach, template-dependent polymerases routinely enable the incorporation of nucleotides bearing small…
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Methods Enzymol. 2026;731:485-501. doi: 10.1016/bs.mie.2026.05.002. Epub 2026 May 25. ABSTRACT Site-specific labeling of antibodies is highly valuable for diagnostic and therapeutic applications. The chemoenzymatic Fc glycan remodeling method provides a platform for site-specific antibody functionalization without perturbing antigen recognition. In this chapter, we describe a one-pot enzymatic method to produce fluorophore- and biotin-labeled antibodies using…
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JACS Au. 2026 Jun 10;6(6):3376-3385. doi: 10.1021/jacsau.6c00382. eCollection 2026 Jun 22. ABSTRACT 16α-Methyl glucocorticoids are a renowned family of steroid molecules with diverse important pharmaceutical applications, and development of innovative, practical, and divergent synthetic routes toward these steroids is highly desirable. Herein, we report a unified stereocontrolled synthesis of five 16α-methyl glucocorticoid pharmaceuticals, namely, (+)-dexamethasone,…
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ACS Synth Biol. 2026 Jun 25. doi: 10.1021/acssynbio.6c00259. Online ahead of print. ABSTRACT Liquid-liquid phase separation (LLPS) utilizes dynamic, membrane-less compartmentalization to spatially organize and control biochemical processes, which advances synthetic biology fields such as synthetic metabolic engineering and artificial cell construction, offering novel solutions to longstanding biomedical and biotechnological challenges. However, the rational design…
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Expanding Steroid Glycodiversity: Tandem Steroid Glucosylation and Acetylation via Enzymatic Cascade
Int J Mol Sci. 2026 Jun 9;27(12):5232. doi: 10.3390/ijms27125232. ABSTRACT Steroid glycosides constitute an important class of bioactive molecules, yet their selective synthesis remains challenging. Here, we established a screening platform for nucleotide sugar-dependent glycosyltransferases (GTs) coupled with sucrose synthase (SuSy) for in situ UDP-glucose regeneration, enabling cost-efficient steroid glucosylation. A library of GTs comprising…
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Microorganisms. 2026 May 24;14(6):1181. doi: 10.3390/microorganisms14061181. ABSTRACT Some microorganisms present in the cultivation environment serve as biocontrol agents and contribute to enhanced mushroom production. However, the native bacteria naturally associated with commercial cultivation bags of Pleurotus ostreatus, as well as their growth-promoting roles and underlying mechanisms, remain poorly understood. This study aimed to identify native…