Bioconjug Chem. 2026 Aug 19;37(8):1530-1536. doi: 10.1021/acs.bioconjchem.6c00344. ABSTRACT DNA-encoded library (DEL) technology has emerged as a powerful platform for small-molecule discovery, in which on-DNA reaction development plays a central role in determining accessible chemical space. Early on-DNA chemistry mainly focused on establishing robust DNA-compatible transformations under mild aqueous conditions but often generated structurally limited libraries.…

J Agric Food Chem. 2026 Aug 19;74(32):25436-25451. doi: 10.1021/acs.jafc.6c04818. ABSTRACT Simultaneously enhancing the thermostability and catalytic activity of acetylxylan esterases (AxEs) remains a significant challenge due to the inherent stability-activity trade-off. Here, a previously uncharacterized CE7 family acetylxylan esterase (TsAxE) from Thermoclostridium stercorarium was identified and engineered through a parallel multidimensional engineering strategy integrating consensus…

ACS Nano. 2026 Aug 18;20(32):22982-23001. doi: 10.1021/acsnano.6c11341. ABSTRACT Diabetic chronic wounds present formidable clinical challenges due to a vicious cycle of methicillin-resistant Staphylococcus aureus (MRSA) infections, persistent hypoxia, and chronic inflammation. We herein report a sprayable and rapidly photo-cross-linkable hydrogel (LMG@GH-MA) that integrates a specially engineered microwave (MW)-responsive luteolin-based nanozyme (Lut-Mn/MnO2) with glucose oxidase (GOx).…

J Vis Exp. 2026 Jul 28;(233). doi: 10.3791/71458. ABSTRACT Industrial biomanufacturing relies on microorganisms to efficiently synthesize target products under engineered conditions. However, wild-type strains often lack the capability to do so due to their native metabolic networks. Therefore, the rapid and accurate screening of high-performance strains from large-scale mutant libraries is a critical step…

Biotechnol Bioeng. 2026 Aug 18. doi: 10.1002/bit.70355. Online ahead of print. ABSTRACT Resolvin E2 (RvE2; 5S,18R-dihydroxyeicosapentaenoic acid) and 17R-resolvin D5 (17R-RvD5; 7S,17R-dihydroxydocosahexaenoic acid) are specialized pro-resolving mediators (SPMs) that actively promote the resolution of inflammation. However, their efficient biocatalytic production remains challenging because of the limited catalytic performance and poor stability of previously reported 5S-lipoxygenases…

J Mol Liq. 2026 Oct 1;459:129832. doi: 10.1016/j.molliq.2026.129832. Epub 2026 Aug 12. ABSTRACT Deep Eutectic Solvents derived from amino acids are considered environmentally friendly because they are biodegradable, recyclable, and exhibit acceptable levels of toxicity. These solvents have various applications in various fields, such as biocatalysis, electrochemistry, extraction, and pharmaceuticals. Glutamine is an amino acid…

Anal Chem. 2026 Aug 18;98(32):23495-23506. doi: 10.1021/acs.analchem.6c01454. ABSTRACT To mitigate the interference from the complex composition of urine from patients with kidney injury and the challenging urinary environment, this study addresses the need for early screening of medium-sized molecular biomarkers in urine. To enable dynamic monitoring of multiple dialysis biomarkers, we propose developing a surface-enhanced…

Angew Chem Int Ed Engl. 2026 Aug 17:e26017. doi: 10.1002/anie.202526017. Online ahead of print. ABSTRACT Cysteine thioaldehydes are putative intermediates in sulfatase-maturing enzyme catalysis and in the biosynthesis of penicillin, coenzyme A and families of ribosomally synthesized and posttranslationally modified peptides (RiPPs). Norrish type-II photolysis of phenacylsulfides gives cysteine thioaldehydes but can be inefficient in…

ChemSusChem. 2026 Aug 27;19(16):e70963. doi: 10.1002/cssc.70963. ABSTRACT Sugar fatty acid esters are bio-based surfactants, but their enzymatic synthesis is limited by poor substrate compatibility in conventional media. In this study, a choline chloride (ChCl)-glucose reactive deep eutectic solvent (RDES) was investigated for lipase-catalyzed transesterification of glucose with vinyl laurate. In this system, glucose, choline, and…