J Am Chem Soc. 2026 Aug 19;148(32):34895-34906. doi: 10.1021/jacs.6c10565. ABSTRACT Sinefungin is a potent nucleoside antimetabolite of S-adenosylmethionine (SAM). Since its discovery in the 1970s, sinefungin has generated significant scientific interest owing to its role as a bioisostere of SAM and its broad range of biological activities. Despite considerable efforts to uncover the enzymes responsible…

bioRxiv [Preprint]. 2026 Aug 1:2026.07.31.742133. doi: 10.64898/2026.07.31.742133. ABSTRACT A protein’s function is derived from its three-dimensional structure and the motions of the atoms about that structure. The detailed characterization of both macromolecular structure and dynamics provides an opportunity for understanding enzyme catalysis, ligand binding, and allostery, along with providing insights into how the function changes…

Front Nutr. 2026 Aug 5;13:1894194. doi: 10.3389/fnut.2026.1894194. eCollection 2026. ABSTRACT BACKGROUND: Excessive intake of added sugars is an important contributor to obesity, type 2 diabetes mellitus (T2DM), dental caries, cardiovascular disease, and other metabolism-related disorders in humans. Stevia-derived sweeteners, particularly high-purity steviol glycosides (SGs), have been widely used in reduced-sugar and sugar-free foods because of…

J Invertebr Pathol. 2026 Aug 19:108718. doi: 10.1016/j.jip.2026.108718. Online ahead of print. ABSTRACT Termites live in dense colonies where frequent social contact can facilitate pathogen transmission, but coordinated social immunity can also limit disease spread. Entomopathogenic fungi (EPF) are particularly relevant to termite pathology because they infect primarily through the cuticle, using adhesion, enzymatic degradation,…

Chempluschem. 2026 Aug;91(8):e70231. doi: 10.1002/cplu.70231. ABSTRACT The multienzyme cascade conversion of CO2 to methanol is a promising route for sustainable chemical synthesis, yet the reduction of formate to formaldehyde remains its major bottleneck. Here, we use quantum chemical calculations to elucidate the catalytic mechanism of formaldehyde dehydrogenase from Burkholderia multivorans (BmFaldDH). The lowest-energy pathway initiates…

J Agric Food Chem. 2026 Aug 19;74(32):25392-25406. doi: 10.1021/acs.jafc.6c03008. ABSTRACT Antagonistic epistasis often limits enzyme engineering by causing activity loss when beneficial mutations are combined. Here, we present a mechanism-guided multidimensional feature analysis (MDFA) strategy integrating rational design, structural partitioning, machine learning, and computational simulations to optimize D-amino acid oxidase (DAAO) for D-phosphinothricin (D-PPT). Channel…

J Agric Food Chem. 2026 Aug 19;74(32):24761-24780. doi: 10.1021/acs.jafc.6c04272. ABSTRACT Nowadays, enzyme engineering has moved from traditional structure-based mutagenesis and directed evolution to data-intensive, AI-assisted design paradigms that involve the rapid discovery and optimization of biocatalysts. Whereas classical approaches relied on rational design and experimental screening, advances in high-throughput sequencing, modeling, and machine learning have…

J Agric Food Chem. 2026 Aug 19;74(32):25369-25379. doi: 10.1021/acs.jafc.5c17370. ABSTRACT Mogroside VI (Mog VI) is a rare triterpene glycoside from Siraitia grosvenorii with promising bioactivities. However, its biosynthesis is limited by a single rate-limiting glycosylation step converting mogroside V, catalyzed by the inherently low-activity plant glycosyltransferase UGT73-327-2. In this study, we applied a structure-guided engineering…

Front Immunol. 2026 Aug 4;17:1877833. doi: 10.3389/fimmu.2026.1877833. eCollection 2026. ABSTRACT BACKGROUND: Gastric cancer (GC) remains a major cause of cancer-related mortality, highlighting the need to clarify the stromal and post-translational mechanisms underlying its progression. METHODS: We integrated single-cell RNA sequencing, independent transcriptomic cohorts, clinical tissue analyses, functional assays, co-immunoprecipitation (Co-IP), multiple immunofluorescence labeling, and a…