Angew Chem Int Ed Engl. 2026 Aug 19:e5014162. doi: 10.1002/anie.5014162. Online ahead of print.

ABSTRACT

Biotin-streptavidin artificial metalloenzymes (ArMs) provide a platform to integrate transition-metal catalysis within a protein-defined microenvironment. Traditional indolone syntheses often rely on transition metal catalysts, high temperatures, and strong oxidants, limiting functional-group tolerance, and sustainability. In this study, we report a visible-light-driven radical cyclization of acrylamides and diazo-esters to indolones mediated by a biotinylated iridium-photocatalyst. Incorporation into engineered streptavidin generates highly active photo-ArMs, enabling reactions under mild aqueous conditions at 0.01 mol% Ir loading, broad substrate scope, and TONs up to 6300. Confinement of the iridium center within the protein binding pocket enhances turnover efficiency, establishing indolone formation as a new transformation accessible via biotin-streptavidin ArMs and highlighting their potential as sustainable platforms for bioactive motif synthesis.

PMID:42615284 | DOI:10.1002/anie.5014162