Chembiochem. 2026 Apr 28;27(8):e70355. doi: 10.1002/cbic.70355. ABSTRACT Artificial enzymes engineered by site-specific incorporation of catalytically active noncanonical amino acids (ncAAs) into protein scaffolds represent a rapidly advancing class of biocatalysts, particularly for chemical transformations lacking natural enzymatic counterparts. Integrating biosynthesis and genetic incorporation of ncAAs has rapidly expanded the toolkit for enzyme design and catalysis.…

Chembiochem. 2026 Apr 28;27(8):e70348. doi: 10.1002/cbic.70348. ABSTRACT This study presents a novel multienzymatic strategy for the efficient and green production of valuable salicylic acid from plant-derived salicin. The biocatalytic route couples β-glucosidase for deglycosylation with laccase and xanthine oxidase (XO) for subsequent oxidation. The primary objective of this approach was to assess the feasibility of…

Brain Behav. 2026 Apr;16(4):e71394. doi: 10.1002/brb3.71394. ABSTRACT BACKGROUND: Multiple sclerosis (MS) is a neurodegenerative disease where dietary intervention has emerged as a potential adjunct treatment. Recently, the modified Paleolithic elimination (MPE) diet, also known as the Wahls diet, and the low-saturated fat (LSF) diet, also known as the Swank diet, were linked to reduced fatigue…

Carbohydr Polym. 2026 Jun 15;382:125240. doi: 10.1016/j.carbpol.2026.125240. Epub 2026 Mar 24. ABSTRACT Pickering interfacial biocatalysis (PIB) often suffers from the mismatch between the neutral pH required for enzyme activity and highly acidic conditions needed for emulsion destabilization during catalyst recycling. To address this, bifunctional starch nanoparticles were designed through sequential TEMPO-mediated oxidation and free-radical graft…

Phys Chem Chem Phys. 2026 Apr 20. doi: 10.1039/d6cp00040a. Online ahead of print. ABSTRACT Organic solvents unlock unique opportunities for biocatalysis but also present challenges, as enzymes often suffer from significant activity loss or even complete inactivation in such environments. In this study, we utilized horseradish peroxidase (HRP) in pure toluene for the oxidative degradation…

Biotechnol J. 2026 Apr;21(4):e70225. doi: 10.1002/biot.70225. ABSTRACT The Bamberger rearrangement is a key route to functionalized aminophenols that are widely used in pharmaceutical synthesis. Hydroxylaminobenzene mutase (HabM) catalyzes a Bamberger-type isomerization in the biosynthesis of the Pranlukast intermediate 3-amino-2-hydroxyacetophenone (3AHAP), but its low catalytic efficiency and unknown structure have limited rational improvement. Here, we combine…

Synth Syst Biotechnol. 2026 Apr 10;14:54-63. doi: 10.1016/j.synbio.2026.03.017. eCollection 2026 Dec. ABSTRACT Plant-derived monoterpenoid α-terpineol has gained significant attention due to its versatile applications in the food, cosmetic and pharmaceutical industries. Microbial biosynthesis offers an environmentally sustainable approach for its commercial-scale production. Here, we developed a combinatorial engineering strategy to enhance α-terpineol production in Saccharomyces…

Biotechnol Adv. 2026 Apr 17:108901. doi: 10.1016/j.biotechadv.2026.108901. Online ahead of print. ABSTRACT As a representative area of industrial biocatalysis, food enzyme engineering plays a critical role in advancing the food industry. Recently, bioinformatics, molecular simulations, and artificial intelligence (AI) have converged into a powerful toolkit for the full spectrum of computational design, from the modification…

Sci Rep. 2026 Apr 18. doi: 10.1038/s41598-026-46062-3. Online ahead of print. ABSTRACT The synthesis of chiral amines is crucial since they are a key component in nearly 40% of top-selling drugs. ω-transaminases are promising biocatalysts for producing these chiral amines. This study reports the isolation of a wild-type Bacillus strain (Bacillus inaquosorum AGSP2) from a…

Appl Microbiol Biotechnol. 2026 Apr 18. doi: 10.1007/s00253-026-13810-4. Online ahead of print. ABSTRACT Inclusion bodies (IBs) in Escherichia coli were once regarded as undesirable aggregates of misfolded proteins, but are now increasingly recognised as functional biomaterials. Here, we show that the N-terminal p40 domain of a lytic polysaccharide monooxygenase from Caldibacillus cellulovorans acts as a…