Proteomics. 2026 Apr 21:e70134. doi: 10.1002/pmic.70134. Online ahead of print. ABSTRACT Comprehensive characterization of proteoforms in complex biological systems remains a significant challenge. While native ion-exchange chromatography (IEC) hyphenated with native mass spectrometry (nMS) is a powerful tool for resolving proteoforms, three critical limitations persist: restricted volatile buffers causing nonlinear pH gradients; insufficient coverage of…

Chem Commun (Camb). 2026 Apr 22. doi: 10.1039/d6cc01143h. Online ahead of print. ABSTRACT Amine dehydrogenases (AmDHs) are a versatile class of enzymes that catalyze the reductive amination of carbonyl compounds with amino donors. The development of engineered AmDHs and the discovery of naturally existing AmDHs represent two powerful strategies for identifying new AmDHs, thereby enabling…

Curr Opin Biotechnol. 2026 Apr 20;99:103495. doi: 10.1016/j.copbio.2026.103495. Online ahead of print. ABSTRACT Advanced alcohols, fatty acids, and sesquiterpenes are promising renewable liquid fuels. This article reviews the latest progress in synthesizing these renewable liquid fuels using microbial cell factories. Beyond conventional metabolic engineering, the use of sustainable carbon sources, the design of artificial pathways,…

Rev Sci Instrum. 2026 Apr 1;97(4):045208. doi: 10.1063/5.0319625. ABSTRACT Stem cell-derived organoid development typically involves an initial gel-free embryoid body (EB) formation step followed by a gel-embedded differentiation phase. Although high-throughput EB formation technologies are well established, the manual gel-embedding step remains a major bottleneck that limits overall throughput. In this study, we introduce OrgDense,…

ACS Chem Biol. 2026 Apr 20. doi: 10.1021/acschembio.5c00763. Online ahead of print. ABSTRACT Uncovering enzymes with promiscuous catalytic activity is significant in biocatalysis to expand their synthetic application toward sustainable chemical transformations, find non-native catalytic activity, and understand enzyme evolution. Mining the whole genome of organophosphate-degrading Sphingopyxis sp. MG to identify potential organochloride-degrading enzymes revealed…

Org Lett. 2026 Apr 20. doi: 10.1021/acs.orglett.6c01113. Online ahead of print. ABSTRACT NADH is a key redox mediator in biology and biocatalysis, yet its catalytic use in non-enzymatic synthetic chemistry remains largely unexplored. Here, we show that NADH can act as a substoichiometric reductive quencher in red-light photoredox catalysis, enabling a redox-neutral C(sp2)-H alkylation of…

Chembiochem. 2026 Apr 28;27(8):e70339. doi: 10.1002/cbic.70339. ABSTRACT This study develops and optimizes a high-cell-density cultivation (HCDC) of E. coli BL21 (DE3) to improve enzyme production utilizing DASGIP multibioreactor systems. By shifting from traditional shake flask methods to HCDC, we observed a 43-fold increase in biomass production, with a comparable mass-specific activity of the cell-free extract.…

Chembiochem. 2026 Apr 28;27(8):e70342. doi: 10.1002/cbic.70342. ABSTRACT Indigo is currently produced from petrochemical sources, which poses significant environmental challenges. Sustainable biotechnological alternatives are therefore highly desirable. Enzymatic synthesis of indigo from L-tryptophan via indole has been demonstrated, but conventional pathways based on flavin-containing monooxygenases require costly coenzymes such as NAD(P)H, limiting their practical applicability. In…

Small. 2026 Apr 21:e73454. doi: 10.1002/smll.73454. Online ahead of print. ABSTRACT Precise control over enzyme structure through carrier design is key for high performance biocatalysis. However, achieving selective enzyme orientation and creating a defined local interface environment remain challenging. In this work, amphiphilic dumbbell-shaped Janus polymer nanoparticles (DS-JPNs) were designed to anchor Aspergillus niger lipase…

Chembiochem. 2026 Apr 28;27(8):e70355. doi: 10.1002/cbic.70355. ABSTRACT Artificial enzymes engineered by site-specific incorporation of catalytically active noncanonical amino acids (ncAAs) into protein scaffolds represent a rapidly advancing class of biocatalysts, particularly for chemical transformations lacking natural enzymatic counterparts. Integrating biosynthesis and genetic incorporation of ncAAs has rapidly expanded the toolkit for enzyme design and catalysis.…