Anal Chim Acta. 2026 Jul 8;1406:345504. doi: 10.1016/j.aca.2026.345504. Epub 2026 Apr 7. ABSTRACT BACKGROUND: β-Galactosidase (β-Gal) serves as an important biomarker for primary ovarian cancer and cellular senescence, as well as a key indicator for Escherichia coli (E. coli). Besides, β-Gal serves as a common reporter enzyme in biosensing systems and is widely applied to…

J Phys Chem Lett. 2026 May 1. doi: 10.1021/acs.jpclett.6c00143. Online ahead of print. ABSTRACT Enzyme catalysis has been shown to depend on distal pathways that channel thermal energy from solvent to the active site. Here, we develop a method to compute these pathways using soybean lipoxygenase (SLO), an extensively studied prototype for dynamical initiation of…

JACS Au. 2026 Apr 8;6(4):2136-2160. doi: 10.1021/jacsau.6c00037. eCollection 2026 Apr 27. ABSTRACT Iron-containing oxygenases play key roles in nature as part of the biosynthesis of natural products as well as the biodegradation of xenobiotics. The biosynthesis of natural products often takes place with high stereo-, chemo-, and regioselectivity, which makes these enzymes of interest to…

Angew Chem Int Ed Engl. 2026 May 1:e11930. doi: 10.1002/anie.202511930. Online ahead of print. ABSTRACT The growing demand for complex molecules continues to drive innovation in organic synthesis, yet challenges in sustainability, selectivity, scalability, and harsh reaction conditions persist. Enzymes offer exquisite chemo-, regio-, and stereoselectivity under mild conditions, while chemocatalysis provides robust and versatile…

J Agric Food Chem. 2026 Apr 30. doi: 10.1021/acs.jafc.6c01422. Online ahead of print. ABSTRACT Chiral pesticides and herbicides (CPHs) show significant enantioselective differences in bioactivity, toxicity, and environmental behavior, making single-enantiomer production highly desirable for better efficacy and lower ecological risk. Among existing methods, lipase-catalyzed kinetic resolution is a more environmentally friendly alternative to chemical…

Nat Commun. 2026 Apr 30. doi: 10.1038/s41467-026-72741-w. Online ahead of print. ABSTRACT Gate residues, acting in concert with distal dynamic networks, are emerging as critical yet underexploited regulators of enzymatic catalysis. Here we show that conformational dynamics analysis of fluoroacetate dehalogenase RPA1163 reveals a gate-based allosteric pair (K181-W185) that governs substrate access and reactivity. Network…

Microb Biotechnol. 2026 May;19(5):e70360. doi: 10.1111/1751-7915.70360. ABSTRACT Polyhydroxyalkanoates (PHAs) are intracellular microbial polyesters whose commercial deployment is strongly influenced by downstream processing costs and environmental burden. Here, we demonstrate a nearly solvent-free, mild aqueous strategy for the recovery of PHAs directly from wet bacterial biomass using crude enzyme systems derived from Tenebrio molitor. Under optimized…

J Am Chem Soc. 2026 Apr 29. doi: 10.1021/jacs.6c05010. Online ahead of print. ABSTRACT Plant biosynthetic genes often scatter in plant genomes. Cytochrome P450 enzymes (CYPs) play pivotal roles in the oxidation of plant terpenoids, such as hydroxylation and oxidation-mediated formation of other functional groups. Hundreds of CYP genes are usually present in a plant…