Protein Sci. 2026 Jun;35(6):e70598. doi: 10.1002/pro.70598. ABSTRACT The bio-based platform chemical 3-hydroxypropionic acid (3-HP) has attracted considerable attention as a precursor for the production of various value-added chemicals, motivating extensive efforts to biosynthesize it using engineered microbial cell factories. Among the known pathways, the cascade of glycerol dehydratase (GDHt) and α-ketoglutaric semialdehyde dehydrogenase (KGSADH) offers…

Bioengineering (Basel). 2026 Apr 16;13(4):465. doi: 10.3390/bioengineering13040465. ABSTRACT Biomineralization has recently emerged as a highly effective strategy for enzyme immobilization. Zeolitic imidazolate frameworks (ZIFs), a subclass of metal-organic frameworks (MOFs), are particularly attractive carriers due to their structural tunability and chemical stability. While ZIF-8 has been extensively studied, its denser and thermodynamically more stable analog…

J Biotechnol. 2026 May 1:S0168-1656(26)00150-1. doi: 10.1016/j.jbiotec.2026.04.024. Online ahead of print. ABSTRACT Nitroaromatic compounds are persistent environmental pollutants that pose significant ecological and toxicological risks. Enzymatic reduction of nitroaromatics to the corresponding aromatic amines offers an efficient and selective degradation pathway, whereas simultaneously yielding valuable intermediates for pharmaceutical and agrochemical synthesis. Here, a nitroreductase (NRBs)…

Water Res. 2026 Apr 27;301:126023. doi: 10.1016/j.watres.2026.126023. Online ahead of print. ABSTRACT Although peroxymonosulfate (PMS)-based oxidation processes are widely used in water treatment, their reliance on continuous external oxidant input results in elevated operational costs and associated environmental impacts. In PMS systems, the degradation of emerging contaminants (ECs) can proceed not only through oxidant-driven reactions…

Arch Biochem Biophys. 2026 May 1:110828. doi: 10.1016/j.abb.2026.110828. Online ahead of print. ABSTRACT (S)-Norcoclaurine synthase ((S)-NCS) catalyzes the stereoselective Pictet-Spengler condensation between dopamine and 4-hydroxyphenylacetaldehyde, forming (S)-norcoclaurine, a central precursor in benzylisoquinoline alkaloid biosynthesis. Beyond its biological role, (S)-NCS is a promising biocatalyst for the sustainable synthesis of chiral tetrahydroisoquinolines, which are valuable scaffolds in…

Nat Commun. 2026 May 2. doi: 10.1038/s41467-026-72684-2. Online ahead of print. ABSTRACT Chemoenzymatic dynamic kinetic resolution (DKR) offers a powerful bridge between chemocatalysis and biocatalysis for the preparation of chiral molecules. However, its broader application has been limited by the incompatibility between racemization and resolution catalysts, where mutual interference often compromises catalytic activity and/or enantioselectivity.…

Acc Chem Res. 2026 May 1. doi: 10.1021/acs.accounts.6c00073. Online ahead of print. ABSTRACT ConspectusEnzymatic catalysis represents a sustainable and selective approach to chemical synthesis, yet its practical implementation is frequently limited by the instability of enzymes under cell-free conditions. Confinement─a principle fundamental to early biochemical evolution─has emerged as a key strategy for maintaining enzymatic activity…

Bioresour Technol. 2026 Apr 29:134751. doi: 10.1016/j.biortech.2026.134751. Online ahead of print. ABSTRACT The instability of traditional glycoside hydrolases in the harsh conditions of pretreatment hinders the industrial scale enzymatic synthesis of the xylo-oligosaccharides using lignocellulosic biomass from agricultural wastes. However, extremophilic xylanases found in thermophiles, halophiles, and acidophiles/alkaliphiles, are intrinsically stable at high temperatures, extreme…