Methods Protoc. 2026 Apr 1;9(2):52. doi: 10.3390/mps9020052. ABSTRACT Pseudomonas aeruginosa causes severe healthcare-associated infections, yet no vaccine has been licenced. To circumvent the antigenic variability of classical surface antigens, we evaluated LolB-an essential outer-membrane lipoprotein whose periplasmic orientation favours T-cell-dominant mechanisms with potential antibody access via outer-membrane vesicles (OMVs) or bacteriolysis. An integrative in silico…

J Agric Food Chem. 2026 Apr 27. doi: 10.1021/acs.jafc.5c15606. Online ahead of print. ABSTRACT Industrial biosynthesis of value-added glycosides in high-viscosity concentrated substrates still faces unprecedented challenges. Herein, manifold computational approaches are combined to rationally engineer the thermal tolerance of sucrose phosphorylase (SPase) for strengthening the performance in high-viscosity system. Through rational combination of ΔΔG,…

Acta Pharm Sin B. 2026 Apr;16(4):2282-2298. doi: 10.1016/j.apsb.2026.01.009. Epub 2026 Jan 13. ABSTRACT The limited repertoire of experimentally validated RNA-targeting nucleases has constrained both mechanistic studies and the efficient discovery of novel enzymes for RNA biotechnology. This challenge is particularly pronounced for prokaryotic Argonaute (Ago) proteins, where the scarcity of confirmed RNA-targeting members and a…

ACS Sustain Chem Eng. 2026 Apr 4;14(15):7205-7216. doi: 10.1021/acssuschemeng.5c13549. eCollection 2026 Apr 20. ABSTRACT Bioplastics such as poly-(ethylene 2,5-furandicarboxylate) (PEF), synthesized from biobased 2,5-furandicarboxylic acid (FDCA), offer a promising alternative to petrochemical-derived plastics. Enzymatic conversion of 5-hydroxymethylfurfural (HMF) to FDCA via hydroxymethylfurfural oxidase (HMFO) is environmentally benign but is limited by catalyst stability. This study…

Front Chem. 2026 Apr 10;14:1792714. doi: 10.3389/fchem.2026.1792714. eCollection 2026. ABSTRACT Quinazolinone derivatives are important nitrogen-containing heterocycles widely used in antitumor agents and functional materials, but their conventional synthesis often relies on noble-metal catalysts and inefficient multistep processes with considerable environmental costs. In this study, we developed a QM/MM hybrid simulation and experimental validation framework to…

Gels. 2026 Apr 3;12(4):306. doi: 10.3390/gels12040306. ABSTRACT Peptide-based supramolecular hydrogels have emerged as promising biomaterials due to inherent biocompatibility, tunable self-assembly, and structural similarity to the extracellular matrix. This work describes the design, synthesis and characterization of a library of symmetrical bolaamphiphiles based on dehydropeptides, systematically varying both the dehydroamino acid residue and the linker.…

Biodes Res. 2025 Aug 29;8(1):100044. doi: 10.1016/j.bidere.2025.100044. eCollection 2026 Mar. ABSTRACT Protein engineering is a powerful tool for applications spanning synthetic biology, biocatalysis, and drug discovery. Recent advances in artificial intelligence (AI), from conventional machine learning (ML) algorithms to large-scale pre-trained protein models, have greatly accelerated enzyme engineering field entering a data-driven era. This review…

Anal Chem. 2026 Apr 27. doi: 10.1021/acs.analchem.6c01132. Online ahead of print. ABSTRACT Sulfur quantum dots (SQDs), as an emerging member of the fluorescent quantum dot family, hold great promise for application in biological analysis and environmental health. However, their intrinsic resistance to fouling in complex matrix pollution is rarely reported. Herein, we report the remarkable…

Chembiochem. 2026 Apr;27(8):e202500896. doi: 10.1002/cbic.202500896. ABSTRACT Enantiopure cyanohydrins are versatile chiral intermediates of several bioactive molecules and life-saving drugs. Consequently, the demand for their efficient and sustainable preparation has grown significantly. We aimed to synthesise them using native and engineered Arabidopsis thaliana hydroxynitrile lyase (AtHNL). Screening of the in-house AtHNL variant library created by saturation…

World J Microbiol Biotechnol. 2026 Apr 25;42(5):236. doi: 10.1007/s11274-026-04975-1. NO ABSTRACT PMID:42033624 | DOI:10.1007/s11274-026-04975-1