Biomolecules. 2026 May 13;16(5):715. doi: 10.3390/biom16050715. ABSTRACT Thermophilic prokaryotic Argonaute proteins (pAgos) have emerged as powerful tools for nucleic acid manipulation, with applications in nucleic acid detection, and DNA assembly. However, their strong dependence on high-temperature catalytic activity limits their utility under moderate conditions. TcAgo, a thermophilic Argonaute nuclease from Thermogladius calderae, exhibits efficient DNA-guided…

Bioengineering (Basel). 2026 May 16;13(5):562. doi: 10.3390/bioengineering13050562. ABSTRACT The advancement of modern regenerative medicine is closely associated with additive technologies that enable the creation of tissue-engineered constructs and personalized bioprostheses. Three-dimensional bioprinting allows precise modeling of tissue architecture and extracellular matrix microstructures. Recent studies demonstrate rapid growth in the use of 3D bioprinting for biomedical…

Molecules. 2026 May 11;31(10):1604. doi: 10.3390/molecules31101604. ABSTRACT The pulping and paper-making (P&P) industry is one of the world’s largest manufacturing sectors, yet it is plagued by high water/energy consumption and massive discharge of highly polluted wastewater. The effluents from pulping, bleaching and papermaking processes are characterized by high chemical oxygen demand (COD), intense color, toxic…

Molecules. 2026 May 11;31(10):1607. doi: 10.3390/molecules31101607. ABSTRACT Caking represents a critical stability challenge for whey permeate powders (WPPs), frequently developing during storage and handling due to moisture-driven structural transformations within the powder bed. This study investigated the physical, morphological, and microstructural characteristics associated with caking in a limited set of industrial WPPs. Five commercial WPP…

Molecules. 2026 May 21;31(10):1769. doi: 10.3390/molecules31101769. ABSTRACT Conifer-derived essential oils have gained attention as versatile natural additives with potential applications in animal production, including influencing microbial processes and supporting environmental sustainability. This study aimed to characterize the chemical composition of selected conifer essential oils (EOs), evaluate their antimicrobial activity against rumen microorganisms in vitro, and…

Microorganisms. 2026 May 8;14(5):1061. doi: 10.3390/microorganisms14051061. ABSTRACT Yeast surface display is a powerful strategy for enzyme immobilization and whole-cell biocatalysis; however, the intracellular processing of heterologous enzymes during secretion and anchoring remains poorly understood. In this study, a GH5 endoglucanase gene (eglS, 1.4 kb) from Bacillus subtilis, originally isolated from a paper mill effluent, was…

Plants (Basel). 2026 May 8;15(10):1438. doi: 10.3390/plants15101438. ABSTRACT Hydrogen sulfide (H2S) functions as a pivotal gaseous signaling molecule in plants, yet its role in promoting crop yield remains elusive. Here, we demonstrate that sodium hydrosulfide (NaHS) application, a donor of hydrogen sulfide (H2S), significantly accelerates growth, promotes flowering, and enhances grain yield in rice (Oryza…

Chembiochem. 2026 May 27;27(10):e70382. doi: 10.1002/cbic.70382. ABSTRACT Enzyme cascade reactions, where the product of one enzyme serves as the substrate for another, are fundamental to cellular metabolism and essential for constructing efficient multi-enzyme systems. Spatial confinement of sequential enzymes can enhance cascade efficiency through proximity effects and substrate channeling, mimicking the organization of natural metabolic…

J Fungi (Basel). 2026 May 8;12(5):350. doi: 10.3390/jof12050350. ABSTRACT This paper systematically reviews the research progress on the physiological functions, gene cloning, classification basis, and expression regulation mechanisms of laccase in edible and medicinal fungi. Laccase is a copper-containing polyphenol oxidase widely distributed in these fungi, characterized by broad-spectrum substrate catalytic activity and redox properties.…

Angew Chem Int Ed Engl. 2026 May 26:e25741. doi: 10.1002/anie.202525741. Online ahead of print. ABSTRACT Hydrophobic tag (HyT)-mediated protein degradation has emerged as a pivotal tool for targeted protein degradation (TPD), yet its underlying degradation mechanism remains incompletely elucidated. Herein, we designed structurally optimized HyT-based degraders by covalently conjugating hydrophobic amino acid tags to ERα-targeting…