Metab Eng. 2026 Jun 9:102486. doi: 10.1016/j.ymben.2026.102486. Online ahead of print. ABSTRACT Bacterial microcompartments (BMCs) are proteinaceous organelles that spatially organize metabolic reactions in bacteria and represent an attractive scaffold for pathway engineering. Here, we present a proof-of-concept in vitro study demonstrating a simple, scalable, and modular BMC shell-based platform for enzyme encapsulation using the…

Adv Sci (Weinh). 2026 Jun 9:e76034. doi: 10.1002/advs.76034. Online ahead of print. ABSTRACT Drought stress severely restricts plant growth and crop productivity. Although numerous transcription factors have been linked to drought responses, the regulatory networks underlying drought tolerance in rice remain incompletely understood. Here, through a CRISPR/Cas9-based genetic screen of a rice mutant population, we…

Adv Sci (Weinh). 2026 Jun 9:e76011. doi: 10.1002/advs.76011. Online ahead of print. ABSTRACT Integration of proteins into metal-organic frameworks (Protein@MOF) offers an effective strategy for protein stabilization across materials and biomedical applications. However, the molecular mechanism of protein-MOF interactions remains poorly understood, limiting rational control over their chemical and physical properties. Here, we develop a…

ChemSusChem. 2026 Jun 15;19(11):e70693. doi: 10.1002/cssc.70693. ABSTRACT The remarkable progress in enzymatic polyester depolymerization has demonstrated the potential of biocatalysis for sustainable polymer recycling. In contrast, enzymatic polyamide (PA) degradation remains poorly understood, largely due to the lack of defined and accessible model substrates. Here, we report a robust solid-phase synthesis strategy for generating defined,…

Angew Chem Int Ed Engl. 2026 Jun 9:e2116720. doi: 10.1002/anie.2116720. Online ahead of print. ABSTRACT Efficient integration of non-natural catalytic motifs into protein scaffolds remains a central challenge in artificial enzyme design. Here we report aniline adenine dinucleotide (AnAD), a NAD+-type synthetic cofactor in which the native nicotinamide is replaced by a catalytically active aniline…

Angew Chem Int Ed Engl. 2026 Jun 9:e8155133. doi: 10.1002/anie.8155133. Online ahead of print. ABSTRACT Photocatalytic biohybrid systems, which interface photosensitizers with whole-cell biocatalysts, represent a platform for sustainable solar-to-chemical CO2 conversion. However, their efficiency is fundamentally constrained by kinetic mismatch between sluggish transmembrane electron injection and uncoupled proton flux required for bioenergetic transduction. This…

ACS Nano. 2026 Jun 9. doi: 10.1021/acsnano.6c04917. Online ahead of print. ABSTRACT Covalent organic frameworks (COFs) with large mesopores beyond their intrinsic micropores are highly desirable for advancing applications in catalysis, biocatalysis, and energy storage, etc. However, the bottom-up construction of such materials by soft template still remains challenging due to poor solvent compatibility, the…

Proc Natl Acad Sci U S A. 2026 Jun 16;123(24):e2534793123. doi: 10.1073/pnas.2534793123. Epub 2026 Jun 8. ABSTRACT Enzymes are fundamental to life, providing the rapid reactions and specificity needed to sustain biological processes. While we have overcome the first major challenge in understanding enzymes-identifying their reaction mechanisms-the second major challenge of quantifying the contributions of…

Proc Natl Acad Sci U S A. 2026 Jun 16;123(24):e2535430123. doi: 10.1073/pnas.2535430123. Epub 2026 Jun 8. ABSTRACT Predicting the substrate reactivity strength for a given biocatalyst remains a central challenge in computational biocatalysis. Here, we present Subdate, a modular workflow that combines descriptor-guided organization of substrate analogs with ab initio metadynamics simulations to prioritize reactive…

Polym Sci Technol. 2025 Jul 31;2(3):136-164. doi: 10.1021/polymscitech.5c00076. eCollection 2026 Mar 24. ABSTRACT Natural enzymes, with their precise structural organization and compartmentalization in metabolic pathways, exhibit remarkable catalytic efficiency, inspiring the design of enzyme-integrated hydrogels for advanced biocatalysis. In this review, we first systematically discuss commonly employed physical strategiesincluding self-assembly, electrostatic adsorption, and direct encapsulationfor…