Talanta. 2026 Jun 11;310:130082. doi: 10.1016/j.talanta.2026.130082. Online ahead of print. ABSTRACT A tandem battery-operated on-site dithizone-functionalized C18 cartridge micro-solid phase extraction and ex-situ small-sized electrothermal vaporization capacitively coupled plasma optical emission spectrometry (μ-SPE-SSETV-μCCP-OES) was developed for river water processing and simultaneous determination of Cd(II), Cu(II), Pb(II) and Zn(II). The cartridge was characterized in terms of…

J Biotechnol. 2026 Jun 13:S0168-1656(26)00186-0. doi: 10.1016/j.jbiotec.2026.06.008. Online ahead of print. ABSTRACT β-Glucosidase is an important industrial enzyme, but its application is often limited by low thermal stability and poor operational reusability. In this study, a magnetic material containing β-glucosidase activity was developed through immobilization of a partially purified fungal enzyme onto modified magnetic nanoparticles,…

Metab Eng. 2026 Jun 12:102488. doi: 10.1016/j.ymben.2026.102488. Online ahead of print. ABSTRACT Saccharomyces cerevisiae is a keystone host for biomanufacturing, yet its metabolic engineering is often complicated by the crosstalk between heterologous pathways and native metabolism. In particular, allocating and balancing the universal reducing equivalents nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) typically requires laborious genetic modifications.…

Angew Chem Int Ed Engl. 2026 Jun 12:e3155545. doi: 10.1002/anie.3155545. Online ahead of print. ABSTRACT Chiral enones are valuable motifs in synthetic intermediates and bioactive molecules, driving significant interest in biocatalysis. Although recent enzymatic desaturation strategies for substituted cyclohexenones provide efficient and highly enantioselective synthetic routes, none offer complementary stereoselectivity. To address this gap, we…

J Nanobiotechnology. 2026 Jun 11. doi: 10.1186/s12951-026-04677-8. Online ahead of print. ABSTRACT Multi-enzyme cascade reactions offer powerful amplification and accelerated biotransformation, yet their implementation in free-solution systems is limited by enzyme instability, diffusion losses, and low coupling efficiency. Here, we reported a spatially confined multi-enzyme cascade nanoreactor (AChE/CHO/HRP@MSN) constructed by co-immobilizing acetylcholinesterase (AChE), choline oxidase…

Int J Mol Sci. 2026 May 27;27(11):4821. doi: 10.3390/ijms27114821. ABSTRACT A series of novel vanillin-derived halolactones bearing a phenolic ring at the β-position were comprehensively evaluated for their antioxidant and anti-inflammatory properties. The most active derivative, trans-4-(4′-hydroxy-3′-methoxyphenyl)-5-(1-iodoethyl)dihydrofuran-2(3H)-one (LV2), exhibited strong antioxidant activity in the ABTS assay (EC50 = 35.65 ± 2.34 μM), comparable with Trolox®…

Polymers (Basel). 2026 May 31;18(11):1364. doi: 10.3390/polym18111364. ABSTRACT This work introduces a textile-based platform for biocatalysis by integrating a copper-based hybrid domain onto aminated/amidine-functionalized acrylic textile (TAC-Cu), producing a functional bio-textile capable of high-performance enzyme immobilization. The textile substrate was chemically modified with ethylenediamine to generate amine/amidine-type functional groups, enabling in situ formation of copper-based…

Chembiochem. 2026 Jun 15;27(11):e70426. doi: 10.1002/cbic.70426. ABSTRACT Formate dehydrogenase (FDH) catalyzes the reversible formate oxidation using NAD+ to yield carbon dioxide and NADH. FDH is widely utilized for cofactor regeneration, enabling the continuous supplying of NADH for chiral chemical biosynthesis. Herein, we employed semi-rational engineering to improve the thermostability of highly catalytic FDH from Bacillus…

J Nat Prod. 2026 Jun 12. doi: 10.1021/acs.jnatprod.6c00490. Online ahead of print. ABSTRACT The tryptophan-to-kynurenine pathway gives rise to neuroactive metabolites associated with neurological, immunological, and metabolic processes, leading to increased interest in the biocatalysis and total synthesis of kynurenine-based drug candidates. Kynurenine scaffolds have been identified in a range of natural product compound classes,…

Org Lett. 2026 Jun 12. doi: 10.1021/acs.orglett.6c02071. Online ahead of print. ABSTRACT While unspecific peroxygenases (UPOs) have been reported to mediate benzylic oxidation of both nitrogen-free and one nitrogen-containing aromatic heterocyclic substrates, the benzylic C-H functionalization of two-nitrogen (diN) heterocycles is rarely reported. Such diN-heterocycles serve as key building blocks for many high-value compounds, including…