J Insect Physiol. 2026 Jul 16:105037. doi: 10.1016/j.jinsphys.2026.105037. Online ahead of print. ABSTRACT Symbiotic microbiota of insects play crucial roles in host development, metabolism, and immunity, but the molecular mechanisms underlying these interactions remain poorly understood, particularly in non-model lepidopteran pests. Traditional germ-free (GF) insect models are primarily generated using antibiotics, which may introduce confounding…

J Agric Food Chem. 2026 Jul 16. doi: 10.1021/acs.jafc.6c07410. Online ahead of print. ABSTRACT A size-matched magnetic core-shell platform (Fe3O4@MnO2@mSiO2) was constructed for immobilizing Candida rugosa lipase (CRL). The flower-like MnO2 interlayer provides chemical shielding, while mesoporous channels were expanded to 6.45 nm to geometrically match CRL (approximately 5 nm). Driven by interfacial activation, the…

Methods Mol Biol. 2026;3019:203-217. doi: 10.1007/978-1-0716-5170-4_16. ABSTRACT Enzymatic biofuel cells are devices that convert chemical energy into electrical energy thanks to electrochemical reactions taking place at the negative and the positive electrodes. They can be used as self-powered biosensors (analyte or inhibitor detection/quantification) or energy storage (biocapacitor or bioenergy generator). Their performances are highly dependent…

Methods Mol Biol. 2026;3019:219-231. doi: 10.1007/978-1-0716-5170-4_17. ABSTRACT Biofuel cells (BFCs) have been gaining popularity as a means of harvesting energy from renewable fuel sources. Even though a wide variety of BFC types have been developed, enzymatic biofuel cells (EBFCs) that employ enzymes as biocatalysts have become appealing technologies due to their ability to convert chemical…

J Agric Food Chem. 2026 Jul 16. doi: 10.1021/acs.jafc.6c01204. Online ahead of print. ABSTRACT PAPS-independent bacterial aryl sulfotransferases (ASTs) do not require the costly and unstable cofactor PAPS like mammalian sulfotransferases. Instead, they use simple aromatic sulfuryl donors. Originally discovered in intestinal bacteria, ASTs display remarkable substrate diversity, catalyzing sulfation of phenols, alcohols, amines, sugars,…

Food Chem. 2026 Jul 7;525(Pt 1):150333. doi: 10.1016/j.foodchem.2026.150333. Online ahead of print. ABSTRACT To overcome the activity-stability trade-off of enzymes in organic solvents, we propose a “Tunnel dynamics guided” engineering strategy. MD simulations revealed that solvents induce a defensive compaction in lipases, creating pathological, narrow tunnels that obstruct catalysis in a dynamic perspective. By targeting…

ACS Appl Mater Interfaces. 2026 Jul 16. doi: 10.1021/acsami.6c04694. Online ahead of print. ABSTRACT We report a novel strategy for constructing a multifunctional catalytic bioreactor by integrating diverse metal-organic frameworks (MOFs) into gelatin via a green coacervation process. A wide range of chemically stable Zr, Fe, Al, and Ti MOFs were incorporated into the gelatin…

Methods Mol Biol. 2026;3019:27-37. doi: 10.1007/978-1-0716-5170-4_4. ABSTRACT Widespread interest in the use of room temperature ionic liquids (RTILs) as solvents in anhydrous biocatalytic reactions has largely been met with underwhelming results. Enzymes are frequently inactivated in RTILs as a result of the influence of the solvent on the enzyme’s microenvironment, be it through interacting with…

ACS Appl Bio Mater. 2026 Jul 15. doi: 10.1021/acsabm.6c00824. Online ahead of print. ABSTRACT Whole-cell biocatalysis of hydrophobic substrates is fundamentally constrained by low solubility and mass transfer limitations, which hinder effective coupling between substrate transport and intracellular metabolism. Herein, we report an interface-regulated nano-bio hybrid system that overcomes these limitations by enhancing substrate accessibility…