Molecules. 2026 Jul 27;31(15):2609. doi: 10.3390/molecules31152609. ABSTRACT Microbial biotransformation of para-hydroxycinnamic acids (pHCAs) such as para-coumaric, caffeic, ferulic and sinapic acids into vinylphenols is catalyzed by phenolic acid decarboxylases (PADs), while reduction to their corresponding aldehydes and alcohols is mediated by carboxylic acid reductases (CARs) and alcohol dehydrogenases (ADHs), respectively. The present study systematically evaluated…

Molecules. 2026 Jul 28;31(15):2628. doi: 10.3390/molecules31152628. ABSTRACT The peripartum period in prolific ewes is associated with increased metabolic demands, and dietary lipid supplementation may increase the energy density of the prepartum diet. This study evaluated the effects of prepartum supplementation with alternative lipid matrices on the physicochemical characteristics and comprehensive fatty acid (FA) profiles of…

Molecules. 2026 Jul 31;31(15):2669. doi: 10.3390/molecules31152669. ABSTRACT We developed a site-directed immobilization strategy exploiting the competitive inhibitor Remazol Brilliant Blue R (RB) to orient horseradish peroxidase (HRP) on functionalized glass surfaces. RB was covalently bound to poly(ethylene-alt-maleic anhydride) (PEMA)-coated substrates, enabling HRP binding via its active site. Subsequent contact with a second PEMA-functionalized surface facilitated…

Int J Mol Sci. 2026 Aug 1;27(15):6884. doi: 10.3390/ijms27156884. ABSTRACT Cannabidiol (CBD) is a bioactive phytocannabinoid with considerable pharmacological potential. However, its limited aqueous solubility and high lipophilicity remain significant barriers to its broader pharmaceutical application. In this study, the enzymatic potential of selected filamentous fungi was investigated as a whole-cell biocatalytic platform for the…

Chem Commun (Camb). 2026 Aug 13. doi: 10.1039/d6cc03182j. Online ahead of print. ABSTRACT Herein, we report a transesterification-based spectroscopic method for distinguishing CD3OD from CH3OH and quantifying ultralow levels of CH3OH in CD3OD with a limit of detection of 0.01% (v/v), operating under mild conditions. PMID:42592855 | DOI:10.1039/d6cc03182j

J Biotechnol. 2026 Aug 12:S0168-1656(26)00228-2. doi: 10.1016/j.jbiotec.2026.08.003. Online ahead of print. ABSTRACT Enzyme immobilization is pivotal for industrial biocatalysis yet often suffers from activity loss, structural distortion, operational instability, and high production costs. This study presents a magnetic bead-T4 capsid biocarrier (MBTCB) platform for efficient immobilization of an engineered tryptophan synthase Pf0A9 to enable sustainable…

J Biol Chem. 2026 Aug 12:113425. doi: 10.1016/j.jbc.2026.113425. Online ahead of print. ABSTRACT Protein arginine methyltransferase 1 (PRMT1) activity is essential for maintaining proper eukaryotic cellular function; however, its dysregulation contributes to the progression of several human diseases and cancers, making PRMT1 an attractive therapeutic target. Efforts to inhibit PRMT1 through active-site-directed competitive strategies have…

Angew Chem Int Ed Engl. 2026 Aug 12:e3915501. doi: 10.1002/anie.3915501. Online ahead of print. ABSTRACT α-Ketoglutarate (α-KG)-dependent nonheme iron enzymes catalyze a diverse array of oxidative transformations essential for natural product biosynthesis. However, the mechanism by which α-KG/Fe-dependent halogenases achieve selective halogenation while circumventing the thermodynamically favored hydroxylation pathway remains a subject of intense debate.…