JACS Au. 2026 Jun 10;6(6):3376-3385. doi: 10.1021/jacsau.6c00382. eCollection 2026 Jun 22. ABSTRACT 16α-Methyl glucocorticoids are a renowned family of steroid molecules with diverse important pharmaceutical applications, and development of innovative, practical, and divergent synthetic routes toward these steroids is highly desirable. Herein, we report a unified stereocontrolled synthesis of five 16α-methyl glucocorticoid pharmaceuticals, namely, (+)-dexamethasone,…

Methods Enzymol. 2026;731:485-501. doi: 10.1016/bs.mie.2026.05.002. Epub 2026 May 25. ABSTRACT Site-specific labeling of antibodies is highly valuable for diagnostic and therapeutic applications. The chemoenzymatic Fc glycan remodeling method provides a platform for site-specific antibody functionalization without perturbing antigen recognition. In this chapter, we describe a one-pot enzymatic method to produce fluorophore- and biotin-labeled antibodies using…

J Org Chem. 2026 Jun 25. doi: 10.1021/acs.joc.6c00980. Online ahead of print. ABSTRACT A chemo-enzymatic approach to enantiopure (S)-linezolid 1 is presented. The key chiral intermediate, (R)-alcohol 4, was obtained via CRL lipase-catalyzed kinetic resolution of (RS)-4, affording an enantiopure (R)-alcohol 4 with 98.5% ees (enantiomeric excess of substrate) and an (S)-ester with 97.1% eep…

Nat Commun. 2026 Jun 24. doi: 10.1038/s41467-026-74245-z. Online ahead of print. ABSTRACT Nature organizes enzymes in micro-compartments to enable efficient biosynthesis, inspiring multienzyme cascades for producing complex molecules. However, practical implementation is often limited by enzyme incompatibility, poor substrate transfer, and inefficient catalyst recycling. Here, we report a living biocatalytic platform that operates as a…

J Am Chem Soc. 2026 Jun 23. doi: 10.1021/jacs.6c06355. Online ahead of print. ABSTRACT 6-sulfo Lewis x-related glycan epitopes, including 6-sulfo Lewis x and its sialylated form, sialyl 6-sulfo Lewis x, constitute an important class of carbohydrate determinants that are widely expressed in human cells. Elucidating the biological roles and structure-function relationships of these glycans…

J Am Chem Soc. 2026 Jun 22. doi: 10.1021/jacs.6c06320. Online ahead of print. ABSTRACT Glycoproteins often exist in nature as heterogeneous glycoforms, making it difficult to obtain pure homogeneous samples for studying the roles of specific N-glycans within a glycoprotein. Despite various chemical and enzymatic methods developed for the synthesis of N-glycans, access to highly…

Org Lett. 2026 Jun 21. doi: 10.1021/acs.orglett.6c01832. Online ahead of print. ABSTRACT A chemoenzymatic strategy to construct aryl-substituted chiral quaternary carbon centers has been established. Directed evolution of P450BM3 enables highly enantioselective epoxidation of tertiary allylic alcohols (up to 99% ee), which is a formidable challenge in chemical synthesis. The resulting chiral epoxides undergo acid-catalyzed…

ACS Synth Biol. 2026 Jun 3. doi: 10.1021/acssynbio.6c00195. Online ahead of print. ABSTRACT Heparin has been the most important drug for treating thrombotic disorders for more than 60 years. However, the traditional production of heparin involves the slaughter of animals. Therefore, there is a demand for the animal-free production of heparin, such as enzymatic synthesis…

J Agric Food Chem. 2026 May 27. doi: 10.1021/acs.jafc.5c12942. Online ahead of print. ABSTRACT Biocatalytic production of human milk oligosaccharides (HMOs) depends on efficient synthesis of the sugar backbone core. Previous studies engineered a D746E variant of a GH20 β-N-acetylhexosaminidase (BbhI) that appears promising for industrial synthesis of the trisaccharide HMO core precursor lacto-N-triose II…

ACS Chem Biol. 2026 May 22. doi: 10.1021/acschembio.6c00013. Online ahead of print. ABSTRACT Thioesterase (TE) domains catalyze peptide release and macrocyclization during nonribosomal peptide biosynthesis, typically via catalytic serine. In contrast, MacB-TE from macolacin biosynthesis employs a rare cysteine nucleophile, raising questions regarding the catalytic requirements of cysteine-dependent peptide cyclases. Here, we report the biochemical…