Biotechnol Bioeng. 2026 Jul 21. doi: 10.1002/bit.70320. Online ahead of print. ABSTRACT Aromatic lactones, including Homogentisate-γ-lactone, 2-coumaronone, and coumarin, have broad applications in pharmaceuticals and agrochemicals, due to their antimicrobial, anti-inflammatory, and antioxidant activities. However, their industrial production relies on multistep chemical synthesis or plant extraction, which suffer from harsh reaction conditions, low efficiency, and…

Org Lett. 2026 Jul 14. doi: 10.1021/acs.orglett.6c02617. Online ahead of print. ABSTRACT Stereoselective syntheses of four dolabellane-type diterpenes, 3,7,12-dolabellatriene, ent-dolabeserpenoic acid A, 3,7,18-dolabellatriene, and 3,4-epoxy-7,18-dolabelladiene were achieved. The syntheses feature chemoenzymatic cyclization of unnatural substrates using a diterpene cyclase, CotB2. Three unnatural substrates bearing synthetically useful functional groups, such as vinyl halide or ketone moieties,…

Angew Chem Int Ed Engl. 2026 Jul 13:e4872790. doi: 10.1002/anie.4872790. Online ahead of print. ABSTRACT A chemoenzymatic strategy is described as a method that can readily provide well-defined α(2,8)- and α(2,9)-linked oligosialic acids of different lengths. It employs the recombinant bacterial sialic acid polymerases, NmC-PST and NmB-PST, in combination with cytidine 5′-monophosphate N-acetylneuraminic acid (CMP-Neu5Ac)…

Angew Chem Int Ed Engl. 2026 Jul 13:e2903805. doi: 10.1002/anie.2903805. Online ahead of print. ABSTRACT A covalent catch-and-release approach is described for the chemoenzymatic synthesis of HS oligosaccharides. It exploits that a glycan modified by a hydrazide tag can under mild acidic conditions efficiently be reacted with an aldehyde-containing resin to give an immobilized hydrazone…

Front Mol Biosci. 2026 Jun 25;13:1846568. doi: 10.3389/fmolb.2026.1846568. eCollection 2026. ABSTRACT Mucins are densely O-glycosylated proteins that establish mucosal barriers, mediate immune recognition, and serve as ligands for host lectins and microbes. However, mucin O-glycan structure-function relationships remain poorly defined due to inherent glycoform heterogeneity. Glycoengineering has emerged as a suite of methodologies enabling construction…

Curr Opin Chem Biol. 2026 Jul 8;93:102722. doi: 10.1016/j.cbpa.2026.102722. Online ahead of print. ABSTRACT Cytochrome P450 enzymes are versatile oxidative enzymes that perform a wide range of oxidation reactions. Here, we summarise recent results showing the widespread role of P450s in the cyclisation of peptides. These enzymes have great potential to act as engineered biocatalysts…

Carbohydr Res. 2026 Jun 26;567:110019. doi: 10.1016/j.carres.2026.110019. Online ahead of print. ABSTRACT Antibody Fc glycosylation critically influences effector functions, motivating the development of chemoenzymatic strategies to generate homogeneous glycoforms. However, limited availability and high cost of mammalian glycosyltransferases remain to be significant constraints. Here, we report the high-yield expression of human and bovine β-1,4-galactosyltransferases (B4GalT1)…

Org Lett. 2026 Jun 29. doi: 10.1021/acs.orglett.6c02562. Online ahead of print. NO ABSTRACT PMID:42374778 | DOI:10.1021/acs.orglett.6c02562

ChemSusChem. 2026 Jul 14;19(13):e70835. doi: 10.1002/cssc.70835. ABSTRACT The development of sustainable biorefineries requires integrated fractionation strategies capable of preserving carbohydrate streams while generating high-value lignin and bioactive fractions. In this study, olive pomace was valorized through a cascade approach combining optimized microwave-assisted extraction (MAE) of phenolic compounds, natural deep eutectic solvents (NADES)-enabled pretreatment, and chemo-enzymatic…

Methods Enzymol. 2026;731:223-252. doi: 10.1016/bs.mie.2026.05.007. Epub 2026 Jun 1. ABSTRACT The ability of native and engineered nucleic acid-processing enzymes to incorporate clickable nucleotide substrates has greatly advanced bioorthogonal labeling of nucleic acids, overcoming the limitations of conventional solid-phase oligonucleotide (ON) synthesis. In this chemoenzymatic approach, template-dependent polymerases routinely enable the incorporation of nucleotides bearing small…