J Am Chem Soc. 2026 Apr 22. doi: 10.1021/jacs.6c03530. Online ahead of print. ABSTRACT Electroenzymatic synthesis, merges the versatility of electrocatalysis with the specificity of enzymology, offering a sustainable strategy for chemical manufacturing. However, the practical application of this approach is often limited by inefficient and incompatible electron transfer between the electrode and enzymes. Here,…

Org Lett. 2026 Apr 17. doi: 10.1021/acs.orglett.6c00887. Online ahead of print. ABSTRACT The availability of an effective ene-reductase (ER) for the synthesis of Sacubitril intermediates represents a major challenge. Herein, we report the engineering of ClER and GoER utilizing three different substrates to synthesize the key Sacubitril precursor. The best performing variant, ClER M3 (M35C/Y76C/Q244G/V289P),…

Molecules. 2026 Apr 7;31(7):1216. doi: 10.3390/molecules31071216. ABSTRACT Selective protein terminal labeling has become essential for system-wide studies of proteolytic mechanisms in disease. These methods enable precise tracking of cleavage dynamics, protease interactions, and cellular networks, offering transformative potential for proteolytic event analysis. This review explores recent advances in N-/C-terminal modification strategies, specifically for the applications…

Org Lett. 2026 Apr 13. doi: 10.1021/acs.orglett.6c00937. Online ahead of print. ABSTRACT Triazolopyridines are an important class of heterocycles in the pharmaceutical industry and materials sciences. In particular, [1,2,3]triazolo[1,5a]pyridines have emerged as stable and versatile diazo compound precursors for performing carbene-mediated transformations. Despite their wide range of applications in chemical synthesis, their preparation is often…

Biomacromolecules. 2026 Apr 11. doi: 10.1021/acs.biomac.5c02511. Online ahead of print. ABSTRACT Resilin, an elastic protein found in insect cuticles, is exceptionally resilient because of its amino acid sequence and dityrosine cross-linking. In this study, the chemoenzymatic synthesis of resilin-mimetic polypeptides based on minimal peptide motifs incorporating glycine, proline, serine, and tyrosine residues is reported. Using…

ACS Chem Biol. 2026 Apr 11. doi: 10.1021/acschembio.6c00132. Online ahead of print. ABSTRACT Ufmylation is a newly identified ubiquitin-like modification of histones and plays important roles in DNA-related processes. Dissecting histone ufmylation pathways necessitates the use of chemically defined proteins to assign their structural and functional consequences; however, the preparation of ufmylated histones has not…

Chembiochem. 2026 Apr 14;27(7):e202500972. doi: 10.1002/cbic.202500972. ABSTRACT Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a well-established target for lowering cholesterol and is abundantly present in the extracellular space. Inhibitors of PCSK9 have achieved marked success in the clinic, but an alternative strategy for therapeutic modulation is emerging through the degradation of PCSK9. This novel strategy…

J Pharm Biomed Anal. 2026 Apr 3;277:117496. doi: 10.1016/j.jpba.2026.117496. Online ahead of print. ABSTRACT The Endoglycosidase CC N180H mutant (Endo-CC N180H) is extensively utilized in the synthesis of homogeneous glycopeptides and glycoconjugates due to its high transglycosylation activity. However, its potential in analytical glycomics remains underexplored. In this study, various structural acceptors were synthesized using…

Org Lett. 2026 Apr 7. doi: 10.1021/acs.orglett.6c00767. Online ahead of print. ABSTRACT We report here the first synthesis of a gem-difluoro spirocyclopropyl phosphonate linked at the anomeric position of a carbohydrate. Three strategies involving carbenes either at the CF2, at the phosphonate, or on the sugar ring were investigated, but only the glycosylidene carbene produced…

Chem Commun (Camb). 2026 Apr 7. doi: 10.1039/d6cc01231k. Online ahead of print. ABSTRACT A COF-OH-Rh catalyst enables formate-driven NADH regeneration via a hydrogen-radical pathway, minimizing byproducts and outperforming hydride transfer. Coupling with glutamate dehydrogenase allows high-yield L-glutamate synthesis. PMID:41944217 | DOI:10.1039/d6cc01231k