Sci Technol Adv Mater. 2026 May 7;27(1):2664156. doi: 10.1080/14686996.2026.2664156. eCollection 2026. ABSTRACT Molecular recognition governs important chemical events in biology from enzyme catalysis to immune defense and cellular signal transduction, where specificity is expressed not only as affinity but also as regulated, state-dependent function. Translating these principles into synthetic materials and systems has stimulated the…

Enzyme Microb Technol. 2026 May 7;199:110897. doi: 10.1016/j.enzmictec.2026.110897. Online ahead of print. ABSTRACT Acetylxylan esterases are accessory enzymes that hydrolyze ester bonds of acetyl groups in acetylated xylan and belong to the carbohydrate esterase family. This work aims to evaluate the immobilization of a recombinant acetylxylan esterase (AxeAN) from Aspergillus nidulans on various supports to…

Proc Natl Acad Sci U S A. 2026 May 19;123(20):e2529979123. doi: 10.1073/pnas.2529979123. Epub 2026 May 12. ABSTRACT Proteins fold through dynamic intermediates that dictate their routes to functional structures, with ensembles predominantly displaying heterogeneity across nanosecond-to-microsecond timescales. Directly observing these states in solution remains challenging as single-molecule methods often require technically demanding microfluidics, surface attachment…

Anal Chem. 2026 May 12. doi: 10.1021/acs.analchem.6c00895. Online ahead of print. ABSTRACT Conventional lateral flow immunoassay (LFIA) based on gold nanoparticles (AuNPs) often suffer from low detection sensitivity. Current signal enhancement strategies, such as metal in situ growth and enzyme catalysis, still face challenges, including poor stability of exogenous chromogenic reagents (prone to aggregation/degradation), uncontrollable…

Bioresour Technol. 2026 May 9:134832. doi: 10.1016/j.biortech.2026.134832. Online ahead of print. ABSTRACT Bioreactors play a crucial role in biocatalysis, particularly where energy efficiency and catalytic stability remain critical challenges. This study reports a continuous-flow photothermal bioreactor based on a MXene/covalent organic framework (MCOF) composite, synthesized in one pot with high surface area and excellent photothermal…

J Chromatogr A. 2026 May 2;1780:467062. doi: 10.1016/j.chroma.2026.467062. Online ahead of print. ABSTRACT Biocatalysis, the use of enzymes to catalyze chemical transformations, is becoming an increasingly important focus in the manufacture of pharmaceutical drug substances. The use of biocatalysts lends marked improvements in process efficiency and sustainability, aligning with industry-wide initiatives for improving greenness. Despite…

Bioelectrochemistry. 2026 May 2;171:109315. doi: 10.1016/j.bioelechem.2026.109315. Online ahead of print. ABSTRACT Deep eutectic solvents (DESs) are a group of emerging solvents for biocatalysis with tailored performance. Herein, we report the usage of hierarchical zeolitic imidazole framework-8 (HZIF-8) and halogenated DES for the immobilization of phenylalanine dehydrogenase. Effects of three different halogenated DESs (I, Cl and…

ACS Omega. 2026 Apr 20;11(17):25014-25024. doi: 10.1021/acsomega.5c09000. eCollection 2026 May 5. ABSTRACT Sulfate-reducing bacteria (SRB) are responsible for biogenic acidification, which negatively impacts oil quality and presents significant economic and environmental challenges for the oil industry. The improper use of biocides often leads to the emergence of biocide-resistant SRB strains, posing additional risks of contaminating…

Infect Drug Resist. 2026 Apr 16;19:568961. doi: 10.2147/IDR.S568961. eCollection 2026. ABSTRACT BACKGROUND: Pseudomonas aeruginosa relies on quorum sensing (QS) to regulate virulence, biofilm formation, and antimicrobial tolerance, making QS inhibition (QSI) an attractive antivirulence strategy. Quercetin is a known QSI-active flavonoid, but its potential as a mushroom-derived therapeutic agent remains underexplored. METHODS: Quercetin was extracted…

J Nat Prod. 2026 May 11. doi: 10.1021/acs.jnatprod.6c00168. Online ahead of print. ABSTRACT Casbanes and the related diterpenoids (e.g., lathyranes, tiglianes, and ingenanes) are yielded from oxidation and oxidation-mediated cyclization of casbene, in which cytochrome P450 enzymes (CYPs) play vital roles. Herein, we characterized four CYPs involved in casbene oxidation from Euphorbia fischeriana Staud. The…