ChemSusChem. 2026 Jun 26;19(12):e70815. doi: 10.1002/cssc.70815. ABSTRACT The development of immobilised biocatalysts for continuous-flow processes is still largely based on laborious trial-and-error screening, and conditions optimised on conventional carriers are often not directly transferable to structured reactors. Here, we introduce a 3D-printed methodology that integrates high-throughput screening of enzyme immobilisation with implementation in a continuous-flow…

Appl Microbiol Biotechnol. 2026 Jun 18;110(1):183. doi: 10.1007/s00253-026-13913-y. NO ABSTRACT PMID:42313152 | DOI:10.1007/s00253-026-13913-y

J Microbiol Methods. 2026 Jun 17:107599. doi: 10.1016/j.mimet.2026.107599. Online ahead of print. ABSTRACT The global escalation of antimicrobial resistance in Staphylococcus aureus (S. aureus) necessitates rapid analytical tools to reliably distinguish between methicillin-susceptible (MSSA) and methicillin-resistant (MRSA). In this study, the surface-enhanced Raman spectroscopy (SERS) was utilized to antibiotic induced biochemical response in MSSA and…

J Genet Eng Biotechnol. 2026 Jun;24(2):100690. doi: 10.1016/j.jgeb.2026.100690. Epub 2026 Apr 7. ABSTRACT Cereibacter sphaeroides 2.4.1, a purple non-sulfur bacterium, is a metabolically versatile organism capable of shifting between aerobic respiration and anaerobic photoheterotrophy. This adaptability makes it an attractive platform for sustainable biocatalysis that harnesses solar energy to drive high-energy biochemical reactions. In this…

Trends Biotechnol. 2026 Jun 17:S0167-7799(26)00184-8. doi: 10.1016/j.tibtech.2026.04.028. Online ahead of print. ABSTRACT Heparin is a critically important clinical anticoagulant. The biosynthesis of heparin using Escherichia coli-based multienzyme cascades represents a promising alternative to animal-derived production. However, efficient deployment of heparin-synthesizing enzymes faces significant challenges, particularly in achieving recombinant expression of functionally active heparin N-deacetylase/N-sulfotransferase (NDST)…

Chembiochem. 2026 Jun 26;27(12):e70403. doi: 10.1002/cbic.70403. ABSTRACT AdoMet-dependent histidine methyltransferases catalyze regioselective methylation of histidine residues in proteins. Nτ-Methylation of His73 in β-actin is catalyzed by histidine methyltransferase SETD3, and represents a unique post-translational modification involved in the regulation of actin polymerization. Likewise, Nπ-methylation of His375 in zinc transporter SLC39A5 is catalyzed by histidine methyltransferase…

Sci Adv. 2026 Jun 19;12(25):eaef2491. doi: 10.1126/sciadv.aef2491. Epub 2026 Jun 17. ABSTRACT The unification of mechanistically distinct oxidative transformations within a single enzyme active site represents a long-standing challenge in biocatalysis. In particular, flavin-dependent oxidative deamination and Baeyer-Villiger oxidation have remained evolutionarily and mechanistically segregated, raising fundamental questions as to whether their catalytic cycles can…

Org Biomol Chem. 2026 Jun 17. doi: 10.1039/d6ob00728g. Online ahead of print. ABSTRACT Chlorothricin is a polyketide-derived natural product isolated from Streptomyces antibioticus. It possesses an elaborate pentacyclic aglycone core which incorporates a spirotetronic acid moiety, linked to a trans-decalin system, embedded within a macrocycle. Using synthetic substrate analogues and purified recombinant proteins, here we…

Synth Syst Biotechnol. 2026 Jun 5;14:429-436. doi: 10.1016/j.synbio.2026.05.003. eCollection 2026 Dec. ABSTRACT Biomanufacturing, with its advantages of mildness, low carbon emissions, and renewability, drives the frontier of green chemistry and sustainable development. However, complex metabolic pathways and intracellular resource competition frequently limit the efficiency and yield of metabolic engineering approaches, particularly for non-natural compounds such…

Mater Today Bio. 2026 Jun 9;39:103336. doi: 10.1016/j.mtbio.2026.103336. eCollection 2026 Aug. ABSTRACT Computationally designed mini-binders have shown great promise, yet current strategies primarily yield molecules targeting a single target. For complex infections, simultaneously engaging multiple pathogenic pathways may offer superior efficacy. Streptococcus equi subspecies zooepidemicus (SEZ) infection, characterized by a highly reproducible and rapid lethal…