Appl Biochem Biotechnol. 2026 Sep 15. doi: 10.1007/s12010-026-05935-z. Online ahead of print. ABSTRACT In present times, globally, solvent-mediated biocatalysis is gaining a pivotal role in sustainable biotechnological solutions for organic synthesis. The versatile green catalyst, laccase, a multicopper oxidoreductase, has gained significant importance in aromatic bioconversion in solvent systems. Laccases portray varied behavioral patterns and…

Proc Natl Acad Sci U S A. 2026 Sep 22;123(38):e2616547123. doi: 10.1073/pnas.2616547123. Epub 2026 Sep 15. ABSTRACT The determination of active-site protonation states is critical for a full mechanistic understanding of enzyme catalysis and inhibition. Here, we employ NMR crystallography-the integrated combination of solid-state NMR spectroscopy, X-ray diffraction, and first-principles computational chemistry-to determine the protonation…

J Agric Food Chem. 2026 Sep 15. doi: 10.1021/acs.jafc.5c08774. Online ahead of print. ABSTRACT The pharmacological activity of protopanaxadiol (PPD) can be enhanced by oxidative modification. Compared with chemical synthesis and biotransformation, synthetic biology offers a more efficient and eco-friendly approach for modifying PPD. In this study, we identified two oxidases from Mucor spinosus. MsSDR3…

ACS Appl Mater Interfaces. 2026 Sep 15. doi: 10.1021/acsami.6c13209. Online ahead of print. ABSTRACT Metal-organic frameworks (MOFs) are widely used for enzyme immobilization due to their tunable pore structures and high stability. However, whether a relatively universal MOF carrier can support diverse enzymatic reactions remains unclear. Herein, we systematically evaluated 120 MOF-enzyme-substrate combinations using ten…

Polymers (Basel). 2026 Sep 1;18(17):2134. doi: 10.3390/polym18172134. ABSTRACT The rapid development of biodegradable polymers has intensified the need for reliable, standardized methodologies that can accurately assess their environmental fate. However, biodegradability is not an intrinsic property of materials, but rather the result of complex interactions between polymer chemistry, physicochemical characteristics, environmental conditions, and microbial activity.…

Int J Mol Sci. 2026 Sep 5;27(17):7928. doi: 10.3390/ijms27177928. ABSTRACT Enantiomerically enriched vanillin-derived cis– and trans-β-aryl-δ-iodo-γ-lactones were synthesized using a chemoenzymatic pathway based on Candida antarctica lipase B (CALB)-catalyzed transesterification of racemic (E)-4-(4′-benzyloxy-3′-methoxyphenyl)but-3-en-2-ol, followed by Claisen rearrangement, hydrolysis, iodolactonization, and deprotection. The trans orientation of substituents on the γ-lactone ring of the target compounds was…

Molecules. 2026 Aug 25;31(17):2967. doi: 10.3390/molecules31172967. ABSTRACT Acorns represent a traditional seasonal feed resource for pigs in Mediterranean production systems; however, comparative information on the nutritional composition and safety of acorns from Central European oak species remains scarce. The present study comprehensively characterized acorns of Quercus robur, Q. petraea, and Q. rubra to assess their…

Angew Chem Int Ed Engl. 2026 Sep 14:e1857480. doi: 10.1002/anie.1857480. Online ahead of print. ABSTRACT Genetic code expansion (GCE) enables the in situ site-specific incorporation of noncanonical amino acids (ncAAs) into proteins. This technology has accelerated the development of next-generation protein therapeutics and expanded the utility of enzymes in advanced biocatalysis. Despite its transformative potential,…