Microb Cell Fact. 2026 Sep 16;25(1):183. doi: 10.1186/s12934-026-03118-3. ABSTRACT BACKGROUND: Alkylamine production depends on ammonium produced via the energy-intensive, fossil-based Haber-Bosch process. Diazotrophic bacteria could offer a biological alternative by supplying nitrogen directly from air to heterotrophic whole-cell biocatalysts for alkylamine production. However, their integration with yeast production systems remains largely unexplored. RESULTS: Here, we…

Angew Chem Int Ed Engl. 2026 Sep 16:e1537547. doi: 10.1002/anie.1537547. Online ahead of print. ABSTRACT Despite their potential synthetic utility, cobamide-dependent MTases are relatively underutilized for biocatalysis. We developed mixed MtaABC/MtgABC enzyme platforms capable of catalyzing methylation of non-native thiol and aniline substrates using unactivated methyl donors like methanol and glycine betaine. This approach allows…

Protein Sci. 2026 Oct;35(10):e70798. doi: 10.1002/pro.70798. ABSTRACT Rigid enzymes catalyze chemical reactions by stabilizing the transition state through a specific conformation. Catalytic residues are precisely positioned, and enzyme dynamics are kept to a minimum. It is thought that conserved residues around the active site (second-shell residues) play a crucial role in positioning catalytic residues. Asp233…

Nat Commun. 2026 Aug 19;17(1):9903. doi: 10.1038/s41467-026-76813-9. ABSTRACT Non-canonical amino acids (ncAAs) are privileged building blocks for the synthesis of natural products, biocatalysts, and drug molecules. Among them, γ-tertiary-nitro-α-amino acids are highly valuable yet remain scarcely explored due to challenges in synthetic accessibility. Although pyridoxal-5′-phosphate (PLP)-dependent enzymes are powerful biocatalysts for ncAA production, no enzymatic…

J Hazard Mater. 2026 Sep 15;517:143643. doi: 10.1016/j.jhazmat.2026.143643. Online ahead of print. ABSTRACT The sulfonamide-degrading monooxygenase sulX plays a dual role in bioremediation and antibiotic resistance, yet its molecular mechanism remains elusive. Here we report crystal structures of sulX in its ligand-free form, as an FMN-bound binary complex, and as ternary complexes with six distinct…

Prep Biochem Biotechnol. 2026 Sep 16:1-19. doi: 10.1080/10826068.2026.2726322. Online ahead of print. ABSTRACT Immobilized enzymes have become central to continuous biotransformations by enabling catalyst recovery, repeated utilization, simplified product separation, and stable long-term reactor operation. This study critically evaluates how immobilization strategies, support architectures, reactor configurations, and reaction media collectively govern enzyme stability, mass transfer,…

Bioprocess Biosyst Eng. 2026 Sep 16. doi: 10.1007/s00449-026-03418-3. Online ahead of print. ABSTRACT Due to the growing demand for eco-friendly nanomaterials, biocatalytic and biogenic nanoparticle production methods have developed as greener alternatives to traditional physicochemical approaches. Traditional methods involve hazardous byproducts, large energy inputs, and toxic chemicals, which pose concerns regarding biocompatibility, scalability, and environmental…

Enzyme Microb Technol. 2026 Sep 14;202:110971. doi: 10.1016/j.enzmictec.2026.110971. Online ahead of print. ABSTRACT Despite rapid advances in biocatalytic polyester depolymerization, enzyme discovery and engineering remain largely focused on polyethylene terephthalate, leaving the molecular basis of activity towards increasingly used biodegradable polyesters poorly understood. Building on the broad substrate range of the polyesterase from the Antarctic…