J Basic Microbiol. 2026 Jul;66(7):e70180. doi: 10.1002/jobm.70180. ABSTRACT Microbial enzymes are central to numerous industrial applications, yet their large-scale production remains constrained by persistent biological and process-level limitations. These include restricted secretion capacity, insufficient operational stability, scale-up inefficiencies, and downstream processing costs that can account for 45-65% of total manufacturing expenses. This review critically examines…

J Agric Food Chem. 2026 Jul 7. doi: 10.1021/acs.jafc.5c17439. Online ahead of print. ABSTRACT (R)-N-(2,6-dimethylphenyl) aminopropionic acid methyl ester ((R)-DMPM) is a key chiral intermediate for the fungicide N-(2,6-Dimethylphenyl)-N-(methoxyacetyl)-alanine methyl ester ((R)-Metalaxyl). The biological function of the (R)-Metalaxyl is heavily dependent on its R-configuration, however, conventional chemical or single-esterase esterification for (R)-DMPM production induces severe…

J Phys Chem B. 2026 Jul 7. doi: 10.1021/acs.jpcb.6c02719. Online ahead of print. ABSTRACT Observed shift from temperature (T)-independent hydrogen tunneling kinetic isotope effects (KIEs) in enzymes to T-dependent KIEs in mutants has been attributed to the donor(D)-acceptor(A) separation effects caused by the weakened protein dynamical compression of D-A distances (DADs) in mutants. To examine…

J Control Release. 2026 Jul 6:115162. doi: 10.1016/j.jconrel.2026.115162. Online ahead of print. ABSTRACT Current gastroesophageal reflux disease (GERD) therapies either systemically suppress gastric acid, risking physiological disruption, or rely on antacid rafts that offer transient, non-responsive neutralization. We address this with a chemistry-centered strategy that spatially localizes proton conversion around the self-positioning raft at the…

Prog Biophys Mol Biol. 2026 Jul 6:S0079-6107(26)00046-5. doi: 10.1016/j.pbiomolbio.2026.07.003. Online ahead of print. ABSTRACT Time-resolved spectroscopy allows direct observation of biomolecular dynamics from femtoseconds to seconds, capturing transient states crucial for understanding protein and enzyme functions. This review summarises key methods-ultrafast optical, infrared, Raman, and X-ray techniques- and their combination with computational simulations for mechanistic…

ACS Synth Biol. 2026 Jul 6. doi: 10.1021/acssynbio.6c00337. Online ahead of print. ABSTRACT Lignin is a sustainable alternative to petroleum as a feedstock for the chemical industry. Emergent strategies for lignin valorization involve tandem processes in which biomass is chemo-catalytically fractionated, followed by bioconversion of the depolymerized lignin by microbial cell factories. A rate-limiting step…

Chembiochem. 2026 Jul 14;27(13):e70456. doi: 10.1002/cbic.70456. ABSTRACT The development of efficient biocatalytic routes for synthesizing primary amines is often hindered by the unfavorable thermodynamic equilibrium of enzymatic transamination. In this study, we present a highly effective bienzymatic cascade coupling the (S)-selective ω-transaminase from Pseudomonas psychrotolerans (PpS-TA) with pyruvate decarboxylase from Zymomonas mobilis (ZmPDC). This integrated…

Bioresour Technol. 2026 Jul 5:135320. doi: 10.1016/j.biortech.2026.135320. Online ahead of print. ABSTRACT Bacillus licheniformis DW2 has emerged as a promising microbial chassis for biosynthesis of industrial enzymes, lipopeptides and biochemicals. However, the lack of dynamic control tools to date has limited its expansion in basic research and industrial applications. Here, a well- performing bifunctional dynamic…

Biomaterials. 2026 Jul 3;335:124417. doi: 10.1016/j.biomaterials.2026.124417. Online ahead of print. ABSTRACT Reactive oxygen species (ROS)-catalytic artificial enzymes have emerged as a promising way to combat malignant tumors, yet their efficacy remains constrained by tumor hypoxia, oxygen dependence, and robust antioxidant defenses. Herein, we report the de novo design of a bioinspired manganese@gold cluster-coordinated covalent organic…

Methods Mol Biol. 2026;3030:299-312. doi: 10.1007/978-1-0716-5249-7_17. ABSTRACT Programmable bacterial adhesion enables applications in biocatalysis, living materials, and targeted drug delivery. This functionality can be achieved through the expression of synthetic adhesins in bacterial cells. These molecules are hybrid proteins composed of four key elements: (i) a signal peptide for secretion; (ii) the beta barrel domain…