ACS Omega. 2026 May 8;11(19):27862-27874. doi: 10.1021/acsomega.5c10884. eCollection 2026 May 19.

ABSTRACT

This investigation, conducted within the framework of the circular economy, employed green coconut waste as a lignocellulosic substrate for the production of xylanase via solid-state fermentation (SSF). Enzymatic yield was optimized through a Box-Behnken experimental design, incorporating initial moisture content, incubation temperature, and fermentation duration as independent variables. Subsequent characterization of the enzyme, performed using a Doehlert design, identified optimal reaction conditions at pH 6.5, 20 °C, and 70 min, under which the xylanase exhibited notable catalytic efficiency and thermal stability. Remarkably, the enzyme demonstrated halotolerance, retaining activity in sodium chloride concentrations of up to 6 mol L^-1, thereby indicating its potential for application under high-salinity industrial conditions. When applied to sugarcane bagasse, the xylanase facilitated a substantial release of reducing sugars, thereby confirming its efficacy in the bioconversion of agro-industrial residues. Collectively, these findings validate the use of statistical optimization strategies and underscore SSF as a sustainable and effective approach for the production of high-performance biocatalysts.

PMID:42179601 | PMC:PMC13191700 | DOI:10.1021/acsomega.5c10884