Discover the Latest In Biocatalysis Research
Encouraging chemists to get connected with recent biocatalysis literature – Updated daily!
November 2025
11-22-2025: Dihydroxy Terpene Synthase: Spatiotemporally Precise Manipulation of Water-Mediated Dihydroxylation via Stepwise Quenching of Carbocations; Zheng-Yu Huang, Kangwei Xu, Wen-Li Li, Chun-Xiu Li, Jiang Pan, Ruibo Wu*, Jian-He Xu* (https://pubs.acs.org/doi/10.1021/jacs.5c19381)
11-20-2025: Biocatalytic Synthesis of N-Protected α-Amino Acids through 1,3-Nitrogen Migration by Nonheme Iron Enzymes; Teng Yuan, Mengxi Zhang, Linqi Cheng, Xinzhe Zheng, Shiyu Jiang, Xiongyi Huang*, Han Xiao* (https://pubs.acs.org/doi/10.1021/jacs.5c11008)
11-20-2025: Biocatalytic- and Chemoproteomic-Guided Discovery of a PHGDH Inhibitor from Chemoenzymatic-Promoted DNA-Encoded Libraries Selection Platform; Yiwei Zhang, Yuqiu Lan, Rufeng Fan, Lei Feng, Guoliang Wang, Xinyuan Wu, Lulu Wen, Zhiqiang Duan, Yueyue Xia, Xudong Wang, Lingrui Zhang, Lu Zhou*, Minjia Tan*, Cangsong Liao*, Xiaojie Lu* (https://pubs.acs.org/doi/10.1021/jacs.5c14634)
11-19-2025: Methionine Salvage Enzyme Uses a Unique Mechanism to Overcome a Challenging Aldose–Ketose Isomerization; Subashi T. Ubayawardhana, Tamal Das, Stephanie A. Corio, Jennifer S. Hirschi*, Andrew S. Murkin* (https://pubs.acs.org/doi/10.1021/jacs.5c13980)
11-19-2025: Photoenzymatic C(sp3)–H Functionalization of 1,3-Dicarbonyls Enables Enantioselective Hydroalkylation of Styrenes; Ermeng Wang, Qiaoyu Zhang, Qinglong Shi*, Xiaoyu Wang, Ting Ma, Yixue Wu, Binju Wang*,Juntao Ye* (https://pubs.acs.org/doi/10.1021/jacs.5c17564)
11-18-2025: Experimental Evidence for Radical-Polar Crossover in Competition with, Rather Than Preceding, Alkene Formation by Microbial Ethylene-Forming Enzyme; Evan J. Burke, Chao Wang, Holly Lussier, Elvira R. Sayfutyarova, Alexey Silakov , Carsten Krebs*, J. Martin Bollinger Jr.*, Jeffrey W. Slater*(https://pubs.acs.org/doi/10.1021/jacs.5c13787)
11-10-2025: Bioinspired Fe Single-Atom Nanozyme Synergizes with Natural NarGH Dimer for High-Efficiency Photobiocatalytic Nitrate Conversion; Jiyong Bian, Jing Zhao, Zixuan Zhang, Dongfei Liu, Xianen Lan, Yang Liao, Xiaoqiang An*, Diannan Lu, Minmin Liang, Ruiping Liu, Junwang Tang*, Huijuan Liu, Jiuhui Qu (https://pubs.acs.org/doi/10.1021/jacs.5c07315)
October 2025
10-24-2025: Stereoselective Photoenzymatic Hydroarylation for the Construction of Quaternary Stereocenters; Felix C. Raps, Chufan A. Jin, Alexandra C. Brown, Damien Sorigué, Todd K. Hyster* (https://pubs.acs.org/doi/10.1021/jacs.5c12440)
10-20-2025: Direct Catalytic Deaminative Cyanation of Aliphatic Primary Amines; Jiang-Hao Xue, Shuang Lin, Jiawu Huang, Taeseok Kang, Bumsu Park, Mark Levin, Myojeong Kim, and Honggen Wang (https://chemrxiv.org/engage/chemrxiv/article-details/68f17790bc2ac3a0e0719d7c) This content is a preprint and has not been peer-reviewed.
10-18-2025: Development of a Manufacturing Route toward AMG 193, an MTA-Cooperative PRMT5 Inhibitor; Athimoolam Arunachalampillai, Prashantha Chandrappa, Richard D. Crockett, Colin S. Gaines, Kelly Hu, Ted Judd, Venkata Chandrasekhar Kommuri, James I. Murray, John Nidhiry, Adrian Ortiz ,Jo Anna Robinson, Andreas R. Rötheli, Robert M. Vernon, Carolyn S. Wei, Shane Wells, Conner V. Wilson, Shuangning Xu, Kumiko Yamamoto*, and Lara E. Zetzsche* (https://pubs.acs.org/doi/10.1021/acs.oprd.5c00310)
10-16-2025: Biocatalytic Stereodivergent Construction of Axially Chiral Tri- and Tetrasubstituted Allenols via Desymmetric Hydroxylation;Xiao-Jian Zhou, Cheng-Cheng Song, An-Ni Wang, Mei-Xia Wang, Bei-Bei Liu, Lu Feng, and Yong-Zheng Chen* (https://pubs.acs.org/doi/10.1021/jacs.5c08146)
10-14-2025: An Expanded Molecular Model for the Activation of Methyl-Coenzyme M Reductase; Elliot B. Shelton, Shadi Yavari, Chau-wen Chou, Nana Shao, Shuning Wang, Evert C. Duin, William B. Whitman, Steven O. Mansoorabadi* (https://pubs.acs.org/doi/10.1021/acs.biochem.5c00494)
10-09-2025: Aminoacyl-tRNA Specificity of a Ligase Catalyzing Non-ribosomal Peptide Extension; Dinh T. Nguyen
Josseline S. Ramos-Figueroa, Alexander A. Vinogradov, Yuki Goto, Mayuresh G. Gadgil, Rebecca A. Splain, Hiroaki Suga, Wilfred A. van der Donk*, Douglas A. Mitchell*(https://pubs.acs.org/doi/10.1021/jacs.5c12610)
10-09-2025: Shaping the Glycan Landscape: Hidden Relationships between Linkage and Ring Distortions Induced by Carbohydrate-Active Enzymes; Isabell Louise Grothaus*, Paul Spellerberg, Carme Rovira, Lucio Colombi Ciacchi (https://pubs.acs.org/doi/10.1021/jacs.5c11504)
10-09-2025: Photobiocatalytic Radical Hydroalkylation with C(sp3)–H Bonds Enabled by Engineered Imine Reductase and Redox Buffering; Bin Chen, Ran Ge, Jinhai Yu, Ruiqi Zhu, Qin Zhu, Jiawei Zhang, Mingfeng Cao, Xiaoqiang Huang* (https://pubs.acs.org/doi/10.1021/jacs.5c10377)
10-08-2025: Directed Evolution of APOX for Proximity Labeling Using Phenols with High Redox Potentials; Sifei Fang, Leslie Acevedo, Alexander Solivais, Xu Zhou, Swapnali S. Patil, Elliot S. Delfosse, Hang Yin, Brian L. Frey, Edwin R. Chapman, Lloyd M. Smith, Jeffrey D. Martell (https://chemrxiv.org/engage/chemrxiv/article-details/68e27cccbc2ac3a0e07d8536) This content is a preprint and has not been peer-reviewed.
10-07-2025: Mechanistic Interrogation of a PQQ and Rare Earth-Dependent Artificial Metalloenzyme; Mohd Taher, Peter J. Thompson, Carlos Nava-Matadamas, Austin T. Bruchs, Jennifer Bridwell-Rabb, Lisa Olshansky (https://pubs.acs.org/doi/10.1021/jacs.5c08540)
10-05-2025: Tryptophanase Mining and Characterization Towards the Biological Production of Indole Derivatives; Ruben M. de Boer, David Harding-Larsen, Stanislav Mazurenko, Ditte Hededam Welner (https://chemrxiv.org/engage/chemrxiv/article-details/68db9276f2aff1677032be9f) This content is a preprint and has not been peer-reviewed.
10-02-2025: Oxidative Rearrangement of Indoles Enabled by Promiscuous Cryptic Halogenation with Vanadium-Dependent Haloperoxidases; Hyung Ji Lee, Carter U. Brzezinski, Sergio A. Solis, Raina S. Semenick, Ana Villalobos Galindo, Sophia G. Barthel, John Bacsa, Kyle F. Biegasiewicz (https://chemrxiv.org/engage/chemrxiv/article-details/68dacf20f416303770df6ce7) This content is a preprint and has not been peer-reviewed.
10-01-2025: Reprogramming the Catalytic Pocket of Baeyer–Villiger Monooxygenase for Environmentally Compatible Synthesis of a Chiral Sulfoxide Pharmaceutical; Chen Zhao, Qiang Geng, Kun Shi, Jiang Pan, HuiLei Yu* (https://pubs.acs.org/doi/10.1021/acscatal.5c06238)
10-01-2025: Late-Stage C(sp³)–H Functionalization of Bioactive Piperazines; Masayuki Wasa, Soichiro Mori , Cunyuan Zhao, Yuankai Wang, Nathan Jackson (https://chemrxiv.org/engage/chemrxiv/article-details/68d694a6f2aff16770b2720d) This content is a preprint and has not been peer-reviewed.
10-01-2025: Connecting chemical and protein sequence space to predict biocatalytic reactions; Alexandra E. Paton, Daniil A. Boiko, Jonathan C. Perkins, Nicholas I. Cemalovic, Thiago Reschützegger, Gabe Gomes & Alison R. H. Narayan (https://doi.org/10.1038/s41586-025-09519-5)
September 2025
09-26-2025: Unspecific Peroxygenases for the Enzymatic Removal of Alkyl Protecting Groups in Organic Synthesis; Lina A. Csechala, Maximilian Wutscher, Verena Scheibelreiter, Stefan Giparakis, Ina Menyes, Thomas Bayer, Christian Stanetty, Florian Rudroff*, Uwe T. Bornscheuer* (https://pubs.acs.org/doi/10.1021/acscatal.5c06385)
09-26-2025: Bio-inspired Deconjugative Isomerization of Borylated Dienoates; Amélia Messara, Byeongseok Kweon, Ferdinand Woge, Constantin G. Daniliuc, Christian Mück-Lichtenfeld, Ryan Gilmour* (https://pubs.acs.org/doi/10.1021/jacs.5c11995)
09-25-2025: A pyridoxal radical carboligase and imine reductase photobiocatalytic cascade for stereoselective synthesis of unnatural prolines; Chen Zhang, Jun Zhou, Binh, Khanh Mai, Zijun Qin, James Finnigan, Samantha Gittings, Peng Liu & Yang Yang (https://www.nature.com/articles/s41557-025-01937-2)
09-22-2025: Chemoenzymatic Decarboxylative Bromination of Acetylenic Acids Using Vanadium-Dependent Chloroperoxidase; Zhigang Zeng, Richa Hu, Jianqun Peng, Yufei Liu, Huanhuan Li*, Wuyuan Zhang* (https://pubs.acs.org/doi/10.1021/acs.orglett.5c03522)
09-22-2025: Lipase-Driven Deconstruction of Polyether Polyurethane Foams through Alcoholysis toward a Closed-Loop Recycling; Riccardo Moro, Daniel Grajales-Hernández, Katarzyna Swiderek, Ion Olazabal, Gabriel Perli, Andrea Rizzo, Mariana Armendáriz-Ruiz, Rut Fernández-Marín, Javier Calvo, Vicent Moliner, Haritz Sardón, Fernando López Gallego (https://chemrxiv.org/engage/chemrxiv/article-details/68caa78f23be8e43d69dddc5) This content is a preprint and has not been peer-reviewed.
09-20-2025: A Versatile Enzymatic Pathway for Modification of Peptide C-Termini; Shravan R. Dommaraju, Sanath K. Kandy, Hengqian Ren; Dominic P. Luciano, Shogo Fujiki, David Sarlah, Huimin Zhao, Jonathan R. Chekan*, Douglas A. Mitchell* (https://pubs.acs.org/doi/10.1021/acscentsci.5c01243)
09-17-2025: High-Efficiency Biocatalytic Degradation of Polyethylene Terephthalate via Engineered Biopolymer-Immobilized Enzymes for Sustainable Plastic Recycling; Wenyan Zhang, Xue Wei, Baotong Zhu* (https://pubs.acs.org/doi/10.1021/acssuschemeng.5c04115)
09-15-2025: Tandem Catalysis Enables the Hydrocarboxylation of γ-Valerolactone with CO2 and H2 to Produce Adipic Acid; Ying Wang
Yanru Zhang, Chenglong Yu, Jia Guo, Jun He, Longbo Zhang, Yanyan Wang, Jianling Zhang, Tianbin Wu, Qingli Qian*, Buxing Han* (https://pubs.acs.org/doi/10.1021/jacs.5c09561)
09-15-2025: Integration of Multiple Enzymes Within Hydrogen-Bonded Organic Frameworks for Efficient Cascade Photocatalytic CO2-to-Methanol Conversion in Water; Jiakang Tang, Yifei Lei, Qingxuan Tang, Shixing Lei, Qiao-Yan Qi, Zhan-Ting Li, Jia Tian (https://doi.org/10.1002/anie.202516599)
09-11-25: Photoenzymatic stereodivergent hydrophosphinylation to access diverse P-chirogenic and P-/C-bichirogenic phosphorus compounds; Kaihao Mou ,Zehua Tian ,Weihua Xu ,Xiuwen Kang ,Zhiguo Wang ,Bei Ding ,Qi Wu (doi:10.26434/chemrxiv-2025-ssqg9) This content is a preprint and has not been peer-reviewed.
09-08-2025: Structural Basis for 3-Amino-3-carboxypropyl Transfer in Nocardicin Biosynthesis; Yaojie Gao, Masayuki Karasawa, Zhiyang Quan, Takahiro Mori, Masahiro Kanaida, Craig A. Townsend, Tohru Terada*, Ikuro Abe*, Takayoshi Awakawa* (https://pubs.acs.org/doi/10.1021/jacs.5c08367)
09-08-2025: Achieving mono-selective palladium(II)-catalysed C–H activation of arenes with protein ligands; Hua-Jin Xu, Zhoulong Fan, Bin-Bin Nian, Chen-Hao Gu, Shou-Jie Shen, Wel Zhang, Yi Hu, and Jin-Quan Yu (https://doi.org/10.1038/s41929-025-01407-5)
09-05-2025: Alcohol Oxidase–Imine Reductase Cascade for One-Pot Chiral Amine Synthesis; Anya Miletic ,Nicholas J Turner , and Rebecca E Ruscoe (doi:10.26434/chemrxiv-2025-9v4xf) This content is a preprint and has not been peer-reviewed.
09-04-2025: Biocatalytic Conversion of Furans into Pyrrolinones Using a Class I Unspecific Peroxygenase; Benjamin Melling, Katy A. S. Cornish, Jared Cartwright, Nicholas P. Mulholland, William P. Unsworth*, Gideon Grogan* (https://pubs.acs.org/doi/10.1021/acscatal.5c05307)
09-01-2025: Covalent Immobilization of Different Enzymes on Hydroxyapatite, an Alternative Green Support; Leonardo Gelati, Antonella Gervasini, Giovanna Speranza, Francesca Paradisi* (https://pubs.acs.org/doi/10.1021/acsomega.5c06430)
August 2025
08-29-2025: Peptidic Tryptophan Halogenation by a Promiscuous Flavin-Dependent Enzyme; Dr. Andrew J. Rice, Mayuresh G. Gadgil, Dr. Paola Bisignano, Richard A. Stein, Dr. Hassane S. Mchaourab, Dr. Douglas A. Mitchell (https://doi.org/10.1002/anie.202509729)
08-26-2025: Transsulfurase-Catalyzed Reversible Modification of C-Terminal Cysteine and Application for Orthogonal Dual Labeling of Proteins; Dr. Bin-Bin Pan, Chi Zhou, Wei-Han Meng, Dr. Ling-Yang Zhang, Jia-Long Zhao, Prof. Xun-Cheng Su (https://doi.org/10.1002/anie.202507661)
08-21-2025: Divergent Synthesis of ΔAA-Peptides Using a Bioorthogonal pro-Amino Acid and Aqueous Flavin Photocatalyst: Green Light Enhances Catalyst Performance and Product Selectivity; Pei-Hsuan Chen, Steven Bloom (https://doi.org/10.1002/anie.202511832)
08-15-2025: A Chemo-Enzymatic Platform for Furanolide Synthesis and Functional Exploration; Xiaoqi Ji, Manuel Einsiedler, Paul M. D’Agostino, Jennifer Herrmann, Rolf Müller, Tobias A.M. Gulder* (https://pubs.acs.org/doi/10.1021/jacs.5c08354)
08-14-2025: Enantioconvergent benzylic C(sp3)‒N coupling with a copper-substituted nonheme enzyme; XUZHONG SHEN, XIAHE CHEN, YIHANG XIAO, JESSE B. BROWN , JAMES G. ZHANG, XINYUAN JI, JINYAN RUI , MARC GARCIA-BORRÀS , YI RAO, YUNFANG YANG, AND XIONGYI HUANG* (DOI: 10.1126/science.adt5986)
08-12-2025: Bioprinting of Nanocellulose Hydrogels for Photobiocatalysis in Continuous Flow Systems; Lenny Malihan-Yap ,
Lisa Schmedler ,Daniel Pint ,Hitesh Medipally ,Florian Lackner ,Simon Fedrigotti ,Rupert Kargl ,Karin Stana Kleinschek ,Robert Kourist ,Heidrun Gruber-Woelfler (doi:10.26434/chemrxiv-2025-v05vd) This content is a preprint and has not been peer-reviewed.
08-11-2025: Biosynthesis of the α-D-mannosidase inhibitor (−)-Swainsonine; Shaonan Liu, Zainab Batool & Yang Hai (https://doi.org/10.1038/s41589-025-01997-y)
08-10-2025: Influence of Diol-Based Deep Eutectic Solvents on Laccase Enzyme Activity and Stability; Madushmita Hatimuria ,
Jyoti Vishwakarma ,Akshara Mohan ,Riya R Krishnan ,Nikesh Chandran C ,Krishna Gavvala ,ASHOK PABBATHI (doi:10.26434/chemrxiv-2025-t3dhx) This content is a preprint and has not been peer-reviewed.
08-07-2025: Insights into the Catalytic Activity of a Metagenome-Derived Urethanase; Katarzyna Swiderek ,Kemel Arafet ,Victor de Sousa Batista ,Daniel Grajales-Hernández ,Fernando López-Gallego ,Vicent Moliner (doi:10.26434/chemrxiv-2025-b9h25) This content is a preprint and has not been peer-reviewed.
08-05-2025: Directed Evolution of Copper-Substituted Nonheme Enzymes for Enantioselective Alkene Oxytrifluoromethylation; James G. Zhang and Xiongyi Huang* (https://pubs.acs.org/doi/10.1021/jacs.4c18532)
08-05-2025: Biocatalytic, Asymmetric Radical Hydrogenation of Unactivated Alkenes; Jaicy Vallapurackal ,Rajib Mandal ,Justin Bossenbroek ,Aris Rubio ,Ethan Poladian ,James Collings ,Cesar Torres ,Matthew Hendrickson ,Julian Morales ,Max Lyons ,Kyle Schultz ,Hannah Shafaat ,Kendall Houk ,Soumitra Athavale (doi:10.26434/chemrxiv-2025-92d97) This content is a preprint and has not been peer-reviewed.
08-05-2025: Imine Reductase-Catalyzed, Radical-Mediated Asymmetric Cyano Group Migration; An Liu and Yang Yang (doi:10.26434/chemrxiv-2025-8h8c2) This content is a preprint and has not been peer-reviewed.
08-04-2025: Chemoenzymatic Skeletal Editing of Natural Product Scaffolds via P450-Controlled Site-Selective Ring Expansion at Aliphatic C─H Sites; John M. Bennett, Andrew R. Bortz, Zheyuan Wang, Muhammed Fastheem, Rudi Fasan (https://doi.org/10.1002/anie.202512576)
08-04-2025: Directed Evolution of Class I Aldolases for Cooperative Photobiocatalytic Enantioselective Radical α-Alkylation of Aldehydes; Quanquan Wang, Zhiyu Bo, Michael S. Bratslavsky, Yang Yang* (https://pubs.acs.org/doi/10.1021/jacs.5c08477)
08-01-2024: Enantioselective Synthesis of Spirocyclic Nitrogen-Containing Heterocycles Catalyzed by an Iridium-Containing Cytochrome; Jingtong Xu, Brandon J. Bloomer, John N. Brunn, Andrew P. Quest, Sukriyo Chakraborty, Joseph E. Schneider, Douglas S. Clark, John F. Hartwig* (https://pubs.acs.org/doi/10.1021/jacs.5c06239)